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Updated: Jun 16, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
An indicator displacement assay with independent dual wavelength emission
Shaohui Zhang1, Timothy E Glass
1Department of Chemistry, 601 S. College Ave, University of Missouri, Columbia, MO 65211.
Abstract:
A multifunctional metallo-receptor was designed with both metal and boronic acid binding groups. A sensor ensemble was prepared using the metallo-receptor and the fluorescent dye ARS. The dye produced two distinct fluorescent bands from interaction with the boronic acid and the metal respectively. Partial displacement of the dye by simple analytes led to different fluorescent signatures than full displacement. This differential response provided easy discrimination of the individual analytes.
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