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Eukaryotic DING proteins are endogenous: an immunohistological study in mouse tissues
Jean-Marc Collombet1, Mikael Elias, Guillaume Gotthard
1Département de Toxicologie, Institut de Recherche Biomédicale des Armées, Centre de Recherche du Service de Santé des Armées, La Tronche, France.
Plos One
|February 18, 2010
Summary
DING proteins are ubiquitous in mouse tissues, suggesting they are endogenous. This study details their presence and cellular localization, crucial for understanding their role in human diseases like rheumatoid arthritis.
Area of Science:
- Molecular Biology
- Immunology
- Genomics
Background:
- DING proteins are characterized by conserved N-terminal sequences.
- Involvement in human diseases such as rheumatoid arthritis, atherosclerosis, and HIV suppression.
- Absence from genomic databases suggests potential prokaryotic contamination, hindering functional studies.
Purpose of the Study:
- To investigate the presence and characteristics of DING proteins in mouse tissues.
- To determine the cellular localization of DING proteins.
- To provide evidence for the endogenous nature of eukaryotic DING proteins.
Main Methods:
- Comprehensive immunological detection of DING proteins in mice.
- Analysis of DING protein isoforms and molecular weights.
- Tissue-specific intracellular localization studies.
Main Results:
- DING proteins were detected in all tested mouse tissues as various molecular weight isoforms.
- Intracellular localization varied by tissue: exclusively nuclear in neurons, and both cytoplasmic and nuclear elsewhere.
- Germ-free mouse plasma showed DING protein levels comparable to wild-type.
Conclusions:
- DING proteins are ubiquitous in mouse tissues, supporting their endogenous origin.
- Precise cellular localization data aids in understanding molecular roles in diseases.
- Provides a foundation for future research on eukaryotic DING proteins.
