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Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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Related Experiment Video

Updated: Jun 16, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

Functional overlap between eIF4G isoforms in Saccharomyces cerevisiae.

Bryan K Clarkson1, Wendy V Gilbert, Jennifer A Doudna

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America.

Plos One
|February 18, 2010
PubMed
Summary
This summary is machine-generated.

Total levels of eukaryotic initiation factor 4G (eIF4G) are critical for protein synthesis, not specific isoforms. This study shows eIF4G levels, not isoform function, dictate mRNA translation efficiency.

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Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Published on: November 9, 2020

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Eukaryotic protein synthesis relies on initiation factor eIF4G, which binds mRNA ends to recruit ribosomes.
  • Many eukaryotes possess multiple eIF4G variants, suggesting diverse roles.

Purpose of the Study:

  • To investigate the functional significance of distinct eIF4G isoforms in budding yeast.
  • To determine if eIF4G isoform levels or specific functions regulate translation initiation.

Main Methods:

  • Gene deletion and homogenic strain construction in Saccharomyces cerevisiae.
  • Analysis of growth rates and global translation initiation.
  • Polysome microarray to assess mRNA translation.

Main Results:

  • Deletion of eIF4G1, but not eIF4G2, impaired growth and translation initiation.
  • mRNAs with longer poly(A) tails were preferentially affected by eIF4G1 loss.
  • Homogenic strains expressing single isoforms at wild-type total eIF4G levels supported normal translation.

Conclusions:

  • Total eIF4G abundance, rather than isoform-specific functions, is the primary determinant of mRNA translational efficiency.
  • Budding yeast growth and translation initiation are sensitive to overall eIF4G levels.