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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles
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The DIANA-mirExTra web server: from gene expression data to microRNA function.

Panagiotis Alexiou1, Manolis Maragkakis, Giorgio L Papadopoulos

  • 1Biomedical Sciences Research Center "Alexander Fleming", Institute of Molecular Oncology, Varkiza, Greece.

Plos One
|February 18, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces DIANA-mirExTra, a web tool for identifying microRNAs (miRNAs) involved in gene deregulation by analyzing 3'UTR motifs. It successfully pinpoints deregulated miRNAs in gene expression datasets.

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Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • High-throughput gene expression studies are crucial for understanding biological conditions.
  • MicroRNAs (miRNAs) are key regulators in development and disease, including cancer.

Purpose of the Study:

  • To develop a computational method for predicting and quantifying miRNA involvement in gene expression deregulation.
  • To introduce DIANA-mirExTra, a user-friendly web server for identifying miRNAs responsible for gene deregulation.

Main Methods:

  • Analysis of overrepresented miRNA-associated motifs in the 3' untranslated regions (3'UTRs) of deregulated genes.
  • Development and implementation of computational approaches using experimental gene expression data.
  • Comparison of motif frequencies between gene sets to identify key miRNAs.

Main Results:

  • The DIANA-mirExTra web server successfully identifies deregulated miRNAs in various experimental datasets.
  • The tool enables the prediction of miRNAs responsible for transcript deregulation.
  • The approach is validated on miRNA overexpression and repression experiment datasets.

Conclusions:

  • DIANA-mirExTra effectively identifies deregulated miRNAs, aiding in understanding gene regulation.
  • The web server integrates with DIANA-mirPath for functional pathway analysis of miRNA targets.
  • Users can filter results based on specific miRNA expression changes for targeted analysis.