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Updated: Jun 16, 2026

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
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Blue fluorescent cGMP sensor for multiparameter fluorescence imaging.

Yusuke Niino1, Kohji Hotta, Kotaro Oka

  • 1Center for Biosciences and Informatics, School of Fundamental Science and Technology, Keio University, Kohoku-ku, Yokohama, Japan.

Plos One
|February 18, 2010
PubMed
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Researchers developed a novel blue fluorescent sensor for cyclic GMP (cGMP). This advancement enables simultaneous monitoring of cGMP, cyclic AMP (cAMP), and Ca(2+) dynamics within single cells, advancing cell signaling research.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Cyclic GMP (cGMP) is a crucial second messenger regulating numerous physiological processes.
  • Existing genetically encoded sensors for cGMP, based on FRET, face limitations due to spectral overlaps when combined with other sensors.
  • A need exists for spectrally distinct biosensors to enable simultaneous monitoring of multiple signaling molecules.

Purpose of the Study:

  • To develop a novel blue fluorescent biosensor for cGMP.
  • To create a spectrally compatible sensor for multiparameter fluorescence imaging.
  • To enable simultaneous monitoring of cGMP, cAMP, and Ca(2+) dynamics in single cells.

Main Methods:

  • Conversion of a FRET-based cGMP sensor into a single wavelength indicator using a dark FRET acceptor.

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  • Development of a blue fluorescent cGMP biosensor.
  • Co-transfection of the blue cGMP sensor with a FRET-based cAMP sensor (CFP/YFP) and loading of a red Ca(2+) probe.
  • Triple-parameter fluorescence imaging.

    • Main Results:

    • A novel blue fluorescent cGMP biosensor was successfully developed.
    • The blue cGMP sensor demonstrated spectral compatibility with CFP/YFP-based cAMP sensors.
    • Successful triple-parameter fluorescence imaging of cGMP, cAMP, and Ca(2+) dynamics in single cells was achieved.

    Conclusions:

    • The developed blue fluorescent cGMP sensor overcomes spectral overlap issues.
    • This sensor facilitates multiparameter fluorescence imaging for studying complex signal transduction networks.
    • The approach using this FRET pair is valuable for individually monitoring the dynamics of multiple second messengers in real-time.