Apoptosis inducing effect of andrographolide on TD-47 human breast cancer cell line
Harjotaruno Sukardiman1, Aty Widyawaruyanti, Sismindari
1Department of Natural Product Sciences, Faculty of Pharmacy Airlangga University, Surabaya, Indonesia.
Abstract:
Andrographolide isolated from Andrographis paniculata Ness (Acanthaceae) at 0.35 mM, 0.70 mM and 1.40 mM induced DNA fragmentation and increased the percentage of apoptotic cells when TD-47 human breast cancer cell line was treated for 24, 48 and 72 h. The results demonstrated that andrographolide can induce apoptosis in TD-47 human breast cancer cell line in a time and concentration-dependent manner by increase expression of p53, bax, caspase-3 and decrease expression of bcl-2 determined by immunohistochemical analysis.
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