Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

We wait for disease to shout-What if we listened when biology whispered?

Cell systems·2026

Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Same author

Developing evidence-based, cost-effective P4 cancer medicine for driving innovation in prevention, therapeutics, patient care and reducing healthcare inequalities.

Molecular oncology·2025

Same author

The role of digital twins in P4 medicine: A paradigm for modern healthcare.

NPJ digital medicine·2025

Same author

Multidomain therapy for Alzheimer's disease: a scoping review of cognitive decline trials.

Molecular neurodegeneration·2025

Same author

A multi-modal medical management and lifestyle intervention increase cerebral blood flow and lowers diabetic risk in persons with early Alzheimer's disease: Mid-trial results from the PREVENTION trial.

Journal of Alzheimer's disease : JAD·2025

Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026

Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026

Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026

Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026

Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026

Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Parallel microfluidic surface plasmon resonance imaging arrays.

Eric Ouellet¹, Christopher Lausted, Tao Lin

¹Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.

|February 18, 2010

Summary

This summary is machine-generated.

This study introduces a novel microfluidic array for high-throughput surface plasmon resonance imaging (SPRi) detection. The device enables rapid, parallel analysis of antibody-protein binding affinities, significantly improving efficiency in biological and medical research.

More Related Videos

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Related Experiment Videos

Last Updated: Jun 16, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Area of Science:

Biotechnology
Analytical Chemistry
Microfluidics

Background:

Surface plasmon resonance imaging (SPRi) is a label-free method for quantifying molecular interactions.
Current SPRi instruments have limitations in parallel analysis and require time-consuming manual serial dilutions for kinetic measurements.

Purpose of the Study:

To develop an integrated microfluidic array for high-throughput SPRi detection.
To enable parallel determination of antibody-protein binding affinities with improved speed and efficiency.

Main Methods:

Utilized soft lithography to create a microfluidic array with 264 element-addressable chambers and microvalves.
Integrated a serial dilution network for simultaneous analysis of multiple analyte concentrations (up to six).
Validated device performance by immobilizing human alpha-thrombin and injecting anti-human alpha-thrombin IgG.

Main Results:

Achieved an equilibrium dissociation constant of 5.0 +/- 1.9 nM for anti-human alpha-thrombin IgG, consistent with literature values.
Demonstrated successful interrogation of multiple ligands against multiple analytes in a single experiment without cross-contamination.
Showcased the capability to analyze up to 264 different immobilized ligands against multiple analytes independently in one experiment.

Conclusions:

The developed microfluidic SPRi array significantly enhances throughput for binding affinity measurements.
This technology is crucial for advancing systems biology and medicine through high-throughput protein analysis.
The device offers a powerful platform for parallel screening of molecular interactions.