Related Experiment Video
Updated: Jun 16, 2026
11:38
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
Published on: May 10, 2016
Cytotoxic phenolic compounds from Chionanthus retusus
Jong Hwan Kwak1, Min Woo Kang, Jong Hwa Roh
1College of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea. jhkwak@skku.edu
Archives of Pharmacal Research
|February 18, 2010
Summary
This study isolated fourteen phenolic compounds from Chionanthus retusus, identifying new compounds for the genus. Several isolated phenolics demonstrated significant anti-proliferative activity against human tumor cell lines.
Area of Science:
- Phytochemistry
- Pharmacology
- Natural Products Chemistry
Background:
- Chionanthus retusus is a plant source of bioactive compounds.
- Phenolic compounds are known for their diverse biological activities.
Purpose of the Study:
- To isolate and identify phenolic compounds from Chionanthus retusus.
- To evaluate the anti-proliferative effects of isolated compounds against human cancer cell lines.
Main Methods:
- Cytotoxicity-guided fractionation and purification of methanol extract.
- Structure elucidation using spectroscopic analysis.
- Anti-proliferative activity assessed via sulforhodamine B assay.
Main Results:
- Fourteen phenolic compounds were isolated, with most being novel to the Chionanthus genus.
- Kaempferol (4), luteolin (8), quercetin (9), and apigenin (10) showed significant anti-proliferative activity (ED50: 1.84–6.35 μg/mL).
- Phillygenin (1), pinoresinol (3), aromadendrin (5), and 3,3',5,5',7-pentahydroxyflavanone (7) exhibited cytotoxic effects.
Conclusions:
- Chionanthus retusus is a valuable source of bioactive phenolic compounds.
- Several isolated phenolics possess potent anti-cancer properties, warranting further investigation.
- This research contributes to the understanding of Chionanthus retusus phytochemistry and potential therapeutic applications.
Related Concept Videos
01:26Anticholinesterase Agents: Poisoning and Treatment
Anticholinesterases, also known as cholinesterase inhibitors, work by blocking the breakdown of acetylcholine, leading to its accumulation in the synaptic cleft. This accumulation indirectly enhances both muscarinic and nicotinic actions. These agents are classified as reversible or irreversible based on their mechanism of action.
Irreversible agents form a strong bond with the cholinesterase enzyme, making it inactive. The breakdown of the phosphorylated enzyme is slower than the...
Irreversible agents form a strong bond with the cholinesterase enzyme, making it inactive. The breakdown of the phosphorylated enzyme is slower than the...
01:27Cytotoxic T Cells-mediated Immune Response
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
01:30The Electron Transport Chain
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...
01:17Antidotes
Antidotes are medicinal substances used to counteract the harmful effects of toxins or drugs in the body. They function in various ways, each uniquely designed to combat specific toxic compounds.
Specific antidotes operate by inhibiting the enzymes that control biochemical pathways, reducing the production of harmful metabolites.
An example of an antidote is atropine, which counteracts the detrimental effects of cholinesterase inhibitors. It achieves this by deactivating muscarinic receptors,...
Specific antidotes operate by inhibiting the enzymes that control biochemical pathways, reducing the production of harmful metabolites.
An example of an antidote is atropine, which counteracts the detrimental effects of cholinesterase inhibitors. It achieves this by deactivating muscarinic receptors,...
01:29Indirect-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship
Indirect-acting cholinergic agonists are agents that interact with the acetylcholinesterase enzyme in the synaptic cleft, preventing the breakdown of acetylcholine into choline and acetate. Consequently, the concentration of acetylcholine in the synaptic cleft increases. These agonists can be classified into reversible and irreversible inhibitors based on their duration of action.
Reversible inhibitors display short to medium durations of action. Short-acting agents include simple alcohols with...
Reversible inhibitors display short to medium durations of action. Short-acting agents include simple alcohols with...