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Related Experiment Videos

Two pathways for GM2(NeuGc) expression in mice: genetic analysis.

M Kono1, M Sekine, K Nakamura

  • 1Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Medical Science.

Journal of Biochemistry
|January 1, 1991
PubMed
Summary

Genetic analysis reveals a single autosomal gene controls GM2(NeuGc) ganglioside expression in WHT/Ht mice tissues. This finding clarifies the genetic basis for ganglioside expression patterns in these mice.

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Area of Science:

  • Biochemistry and Genetics
  • Glycosphingolipid Metabolism
  • Mouse Models in Research

Background:

  • WHT/Ht mice exhibit a deficiency in GM2(NeuGc) and GM1(NeuGc) in liver and erythrocytes due to a defect in the Ggm-2 gene.
  • Previous studies indicated WHT/Ht mice express GM2(NeuGc) in tissues other than liver and erythrocytes, but the genetic control was unclear.

Purpose of the Study:

  • To determine if the lack of detectable GM2(NeuGc) in specific tissues of WHT/Ht mice is genetically controlled.
  • To investigate the inheritance pattern of GM2(NeuGc) expression in WHT/Ht mouse progeny.

Main Methods:

  • Analysis of ganglioside expression in progeny from a backcross mating between (BALB/c X WHT/Ht)F1 and WHT/Ht mice.
  • Phenotypic analysis of a GM2(NeuGc) congenic mouse model (WHT.C).

Related Experiment Videos

  • Segregation analysis of GM2(NeuGc) expression in liver, lung, and kidney tissues.

    • Main Results:

    • Backcross mice segregated into two types based on GM2(NeuGc) expression in liver, lung, and kidney (ratio 42:60).
    • This segregation pattern indicates genetic determination by a single autosomal gene.
    • No recombination was detected for GM2(NeuGc) expression in the analyzed tissues among 102 backcross mice.

    Conclusions:

    • A single autosomal gene controls the expression of GM2(NeuGc) ganglioside in the liver, lung, and kidney of WHT/Ht mice.
    • The GM2(NeuGc) congenic mouse (WHT.C) expresses detectable levels of GM2(NeuGc) across multiple tissues, including liver and erythrocytes.