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Identification of a unique membrane receptor for adenosine 5',5"'-P1,P4-tetraphosphate
R H Hilderman1, M Martin, J K Zimmerman
1Department of Biological Sciences, Clemson University, South Carolina 29634-1903.
The Journal of Biological Chemistry
|April 15, 1991
Abstract:
Adenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) has been implicated as a modulator of cell stress. We have performed binding studies which indicate that membranes from all tissues tested bind tritium-labeled Ap4A. The characteristics of Ap4A binding were determined on brain membrane homogenates after development of an optimized in vitro filter-binding assay. Ap4A binding is specific for adenylated dinucleotides and for the length of the phosphate bridge. A Kd of 0.71 microM for Ap4A was determined.