Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ontogenesis of glomerular basement membrane: structural and functional properties.

M Desjardins1, M Bendayan

  • 1Département d'anatomie, Université de Montréal, Québec, Canada.

The Journal of Cell Biology
|May 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COVID-19: semen impairment may not be related to the virus.

Human reproduction (Oxford, England)·2021
Same author

What could cause the long-term effects of COVID-19 on sperm parameters and male fertility?

QJM : monthly journal of the Association of Physicians·2021
Same author

Covid-19 and impairment of spermatogenesis: What if fever was the only cause?

EClinicalMedicine·2020
Same author

Predicting sperm extraction in non-obstructive azoospermia patients.

Human reproduction (Oxford, England)·2020
Same author

COVID-19 and andrology: Recommendations of the French-speaking society of andrology (Société d'Andrologie de langue Française SALF).

Basic and clinical andrology·2020
Same author

Impact of reduced incubation times on culture and susceptibility testing of urine cultures incubated in the BD Kiestra ReadA Compact incubators.

Diagnostic microbiology and infectious disease·2019

Renal basement membrane composition changes during maturation, affecting glomerular filtration. Type IV collagen and entactin distribution shifts, enabling permselectivity acquisition in the kidney.

Area of Science:

  • Nephrology
  • Cell Biology
  • Biochemistry

Background:

  • Renal basement membranes (RBMs) are crucial for kidney function.
  • Understanding RBM maturation is key to kidney development and disease.

Purpose of the Study:

  • To investigate the spatiotemporal changes in RBM composition during renal maturation.
  • To correlate these changes with the development of glomerular filtration properties.

Main Methods:

  • Protein A-gold immunocytochemistry to localize collagen IV chains (alpha 1(IV), alpha 2(IV), alpha 3(IV)) and entactin.
  • Morphometrical analysis of RBM components.
  • Tracking endogenous serum albumin distribution as a marker for permselectivity.

Main Results:

Related Experiment Videos

  • Heterogeneity in RBM composition emerges during maturation.
  • Alpha 1(IV) and alpha 2(IV) collagen chains decrease and shift to the endothelial side.
  • Alpha 3(IV) collagen increases and localizes centrally; entactin remains distributed throughout.
  • Serum albumin distribution shifts, indicating progressive glomerular permselectivity acquisition.

Conclusions:

  • Maturation involves significant remodeling of RBM constituents.
  • These compositional and organizational changes are essential for establishing glomerular filtration function.
  • The study provides insights into the molecular basis of kidney development and permselectivity.