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A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
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Cloning of PRL and VIP cDNAs of the Java sparrow (Padda oryzivora)

Gen Hiyama1, Tsukasa Sato, David Zadworny

  • 1Laboratory of Animal Genetics and Breeding, Azabu University, Sagamihara, Japan.

Animal Science Journal = Nihon Chikusan Gakkaiho
|February 19, 2010
PubMed

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The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter telomeres than other...
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