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Embryo development of porcine oocytes after injection with miniature pig sperm and their extracts
1Suzuka Kaisei General Hospital, Suzuka, Japan.
Abstract:
This study examined embryo development of porcine oocytes after microinjection of sperm extracts (SE) in porcine intracytoplasmic sperm injection (ICSI). SE was prepared from miniature pig sperm by a nonionic surfactant, and various concentrations (0.02, 0.04 and 0.08 mg/mL) of SE were injected into the matured oocytes with a first polar body. In the pronuclear stage, the rate of oocytes with two pronuclei and a second polar body (21.4%) in the sperm and SE (0.04 mg/mL) injection group was significantly higher (P < 0.05) compared to other groups. The rate of 2-4-cell stage in sperm and SE (0.04 mg/mL) injection group was 38.1%, and it was significantly higher than that in the sperm injection group (22.9%). The rate of blastocyst stage in sperm and SE (0.04 mg/mL) injection group was 21.4%, the value was significantly higher than those in SE (0.08 mg/mL) injection group (0%), sperm injection group (5.7%), and sperm and SE (0.08 mg/mL) injection group (2.6%). These results suggest that SE induces activation of porcine oocytes and their further embryonic development, and that SE is effective for porcine ICSI.
Insights
Sperm extracts enhance porcine oocyte activation and embryo development during intracytoplasmic sperm injection (ICSI). The optimal concentration of sperm extracts (SE) improved developmental rates to the 2-cell and blastocyst stages.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Animal Science
Background:
- Intracytoplasmic sperm injection (ICSI) is a key technique in assisted reproduction.
- Porcine oocyte activation is crucial for successful embryonic development after ICSI.
- Sperm extracts (SE) have shown potential in inducing oocyte activation.
Purpose of the Study:
- To investigate the effect of sperm extracts (SE) on porcine oocyte activation and embryonic development.
- To determine the optimal concentration of SE for improving ICSI outcomes in pigs.
- To evaluate the efficacy of SE in enhancing porcine embryo development.
Main Methods:
- Mature porcine oocytes were microinjected with sperm extracts (SE) at varying concentrations (0.02, 0.04, 0.08 mg/mL) combined with sperm.
- Control groups included sperm injection alone and SE injection alone.
- Oocyte activation, pronuclear formation, and subsequent embryonic development stages (2-4 cell, blastocyst) were assessed.
Main Results:
- The combination of sperm and 0.04 mg/mL SE significantly increased the rate of oocytes reaching the pronuclear stage compared to other groups.
- The 2-4 cell stage development rate was significantly higher in the sperm and 0.04 mg/mL SE group (38.1%) than in the sperm injection group (22.9%).
- Blastocyst formation rate was significantly higher in the sperm and 0.04 mg/mL SE group (21.4%) compared to other SE concentrations and the sperm injection group.
Conclusions:
- Sperm extracts (SE) effectively induce porcine oocyte activation and promote subsequent embryonic development.
- The concentration of 0.04 mg/mL SE is optimal for enhancing porcine ICSI outcomes.
- SE holds promise as a valuable supplement for improving the efficiency of porcine ICSI procedures.
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