Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Novel UHMWPE sleeve for stabilizing loose suture anchors in rotator cuff repair.

Archives of orthopaedic and trauma surgery·2026

Same author

Nanobubbles, not microbubbles, enable ultrasound visualization of the perivascular space: A validation and focused ultrasound modulation study.

Ultrasonics·2026

Same author

A novel UHMWPE anchor for improving the pullout resistance of pedicle screws.

Frontiers in bioengineering and biotechnology·2026

Same author

Comprehensive evaluation of cryopreserved aortic homografts: Long-term mechanical and histological preservation across human and porcine models.

Journal of tissue engineering·2025

Same author

Multiple large language models versus clinical guidelines for postmenopausal osteoporosis: a comparative study of ChatGPT-3.5, ChatGPT-4.0, ChatGPT-4o, Google Gemini, Google Gemini Advanced, and Microsoft Copilot.

Archives of osteoporosis·2025

Same author

Investigation into the synergistic effect of atorvastatin combined with ultrasound stimulation for anti-glioma therapy.

Oncology letters·2025

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

Polycation/DNA complexes coated with oligonucleotides for gene delivery.

Yi-Chen Chung¹, Wen-Yuan Hsieh, Tai-Horng Young

¹Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan.

|February 19, 2010

Summary

Researchers developed novel ternary nanoparticles for gene delivery. These negatively charged, oligonucleotide-coated nanoparticles show efficient cellular uptake, high gene expression, and low toxicity in various cell types, paving the way for advanced gene therapy.

More Related Videos

Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Related Experiment Videos

Last Updated: Jun 16, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Area of Science:

Biotechnology
Nanomedicine
Gene Therapy

Background:

Gene delivery systems are crucial for therapeutic applications.
Developing safe and efficient non-viral vectors remains a challenge.
Polyallylamine-histidine (PAA-HIS)/DNA complexes are investigated for gene delivery.

Purpose of the Study:

To engineer ternary nanoparticles with a negative surface charge for enhanced gene delivery.
To investigate the cellular uptake mechanism and efficiency of these novel nanoparticles.
To evaluate the cytotoxicity and transfection capabilities of the developed gene delivery system.

Main Methods:

Preparation of ternary nanoparticles by coating single-stranded oligonucleotides onto PAA-HIS/DNA complexes.
Characterization of nanoparticle size and surface charge using dynamic light scattering and zeta potential measurements.
Assessment of cellular internalization, gene expression, and cytotoxicity in HeLa cells and other cell lines (HEK-293, HepG2, Hs68).

Main Results:

Ternary nanoparticles exhibited a negative surface charge (-27 mV) and a size of approximately 100 nm.
The negatively charged nanoparticles were efficiently internalized by HeLa cells, leading to high gene expression with low cytotoxicity.
Gene expression was significantly inhibited by other polyanions, suggesting a specific receptor-mediated uptake mechanism.
The oligonucleotide-coated complexes demonstrated resistance to erythrocyte agglutination and serum inhibition.
Effective transfection was observed in multiple cell types, including HEK-293, HepG2, and Hs68 cells.

Conclusions:

Coating PAA-HIS/DNA complexes with specific oligonucleotides creates safe and efficient gene delivery nanoparticles.
The negatively charged surface and receptor-mediated uptake contribute to the enhanced transfection efficiency.
This nanotechnology holds promise for advancing safe and effective gene therapy applications.