Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HIV antigen detection in circulating immune complexes.

L Kestens1, G Hoofd, P L Gigase

  • 1Institute of Tropical Medicine, Antwerpen, Belgium.

Journal of Virological Methods
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic value of geriatric and cardiac parameters for one-year mortality in older heart failure patients. A multicentre, observational, prospective study.

Acta clinica Belgica·2024
Same author

A case of disseminated tuberculosis during a twin pregnancy following in vitro fertilization.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2022
Same author

Use of resuscitation promoting factors to screen for tuberculosis infection in household-exposed children in The Gambia.

BMC infectious diseases·2020
Same author

Thirty-five years of CD4 T-cell counting in HIV infection: From flow cytometry in the lab to point-of-care testing in the field.

Cytometry. Part B, Clinical cytometry·2016
Same author

Tuberculin skin test conversion among HIV patients on antiretroviral therapy in Uganda.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2013
Same author

Association between herpes simplex virus type 2 and HIV-1 in a population of married couples from Dakar, Senegal.

International journal of STD & AIDS·2012
Same journal

Epidemiological characteristics of respiratory syncytial virus in children during 2021-2024.

Journal of virological methods·2026
Same journal

Beyond strain-specific immunity: Conserved antigenic targets, emerging platforms, and translational challenges in universal influenza and pan-coronavirus vaccine development.

Journal of virological methods·2026
Same journal

Analytical characteristics of the NeuMoDx™ SARS-CoV-2 assay and clinical agreement with the BD MAX system.

Journal of virological methods·2026
Same journal

Development of a real-time PCR assay for tracking the spatiotemporal dynamics of a cyanophage and its Synechococcus host in waters of shrimp aquaculture ecosystems.

Journal of virological methods·2026
Same journal

A universal RdRp-targeted primer set for broad, non-specific detection of Quinvirinae and other Betaflexiviridae infecting stone fruits.

Journal of virological methods·2026
Same journal

Development of a multiplex real-time PCR method for detecting immunosuppressive viruses and its preliminary application in broilers.

Journal of virological methods·2026
See all related articles

A new method effectively releases HIV antigen (Ag) from immune complexes (IC) in patient serum. This technique improves detection of HIV Ag, particularly in asymptomatic individuals and across all infection stages.

Area of Science:

  • Immunology
  • Virology
  • Biochemistry

Background:

  • Immune complexes (IC) containing HIV antigen (Ag) and antibody (Ab) can impact diagnostic accuracy.
  • Effective dissociation of IC is crucial for reliable detection of viral components.

Purpose of the Study:

  • To evaluate methods for releasing HIV Ag from artificial and patient-derived IC.
  • To establish an effective protocol for detecting HIV Ag in the presence of IC.

Main Methods:

  • Evaluation of various dissociation techniques for HIV IC.
  • Optimization of a method involving polyethylene glycol precipitation followed by acid-SDS treatment.
  • Detection of HIV Ag in serum from HIV Ab-positive individuals before and after IC dissociation.

Related Experiment Videos

Main Results:

  • A method combining polyethylene glycol precipitation and acid-SDS treatment proved highly effective for HIV IC dissociation.
  • HIV Ag was detected in 47% of asymptomatic HIV Ab-positive subjects after IC dissociation.
  • Prevalence of IC was observed across all infection stages, increasing in later stages.

Conclusions:

  • The developed method reliably dissociates HIV IC, enhancing HIV Ag detection.
  • Improved detection of HIV Ag in asymptomatic individuals and across various disease stages is possible.
  • Immune complex detection offers valuable insights into HIV infection dynamics.