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Three-phase partitioning of hydrolyzed levan.

Cynthia Gisele de Oliveira Coimbra1, Carlos Edison Lopes, Glícia Maria Torres Calazans

  • 1Department of Antibiotics, Federal University of Pernambuco, 50.670-901-Recife-PE, Brazil. cynelesig@yahoo.com.br

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Three-phase partitioning (TPP) effectively separates fructooligosaccharides (FOS) from levan solutions. This method enhances the yield of low-molecular-weight sugars, offering potential applications in sugar separation.

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Area of Science:

  • Biochemistry
  • Separation Science

Background:

  • Fructooligosaccharides (FOS) are smaller molecules produced during levan synthesis or via levan hydrolysis.
  • Three-phase partitioning (TPP) is a technique utilizing t-butanol and ammonium sulphate for polysaccharide separation from aqueous solutions.

Purpose of the Study:

  • To evaluate the efficacy of three-phase partitioning (TPP) for separating levan and fructooligosaccharides (FOS) from aqueous solutions.
  • To investigate the yield and characteristics of FOS fractions obtained using TPP.

Main Methods:

  • Fructooligosaccharides (FOS) were obtained from Zymomonas mobilis levan through acid hydrolysis and ethanol fractionation.
  • Three-phase partitioning (TPP) was applied to separate levan and FOS from aqueous solutions.
  • Fractions were analyzed for yield and separation efficiency.

Main Results:

  • The TPP method yielded higher amounts of low-molecular-weight FOS fractions compared to native levan.
  • The F-90 fraction showed the highest yield among the separated fractions.
  • The TPP technique was less effective in visualizing an intermediate phase for the lightest fraction (F>90) not precipitated by ethanol.

Conclusions:

  • Three-phase partitioning (TPP) demonstrates potential for separating low-molecular-weight sugars like FOS from levan.
  • The TPP method can be optimized to improve the yield of specific FOS fractions.