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Ultrastructural changes and cyclic AMP in frog oxyntic cells.

K S Carlisle, C S Chew, S J Hersey

    The Journal of Cell Biology
    |January 1, 1978
    PubMed
    Summary
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    Theophylline stimulates frog gastric oxyntic cells, increasing plasma membrane surface area and altering mitochondrial structure. This theophylline-induced effect is mediated by cyclic AMP, not acid production itself.

    Area of Science:

    • Physiology
    • Biochemistry
    • Cell Biology

    Background:

    • Oxyntic cells are responsible for gastric acid secretion.
    • Understanding the morphological changes during acid secretion is crucial.
    • Histamine H2-receptor antagonists like metiamide serve as controls.

    Purpose of the Study:

    • To investigate the morphological and biochemical changes in frog gastric mucosa during acid secretion.
    • To elucidate the role of theophylline in stimulating acid production and associated cellular changes.
    • To determine the involvement of cyclic AMP in theophylline-induced morphological alterations.

    Main Methods:

    • Utilized an in vitro frog gastric mucosa model.
    • Employed physiological, biochemical, and ultrastructural analyses.

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  • Used theophylline to stimulate acid production and metiamide as a control.

    • Main Results:

    • Theophylline stimulation led to a 10-fold increase in plasma membrane surface area.
    • Distinct changes in mitochondrial cristae conformation were observed.
    • Theophylline-dependent surface area changes were independent of acid production and oxidative metabolism.
    • Increased cyclic AMP levels correlated with observed morphological changes.

    Conclusions:

    • Theophylline induces significant morphological changes in oxyntic cells, including increased plasma membrane surface area.
    • Cellular cyclic AMP levels are likely regulators of theophylline-induced surface area changes.
    • Acid production and oxidative metabolism are not essential for these specific theophylline-driven morphological alterations.