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Related Experiment Video

Updated: Jun 16, 2026

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

Rhadinovirus vector-derived human telomerase reverse transcriptase expression in primary T cells.

T Toptan1, A Ensser, H Fickenscher

  • 1Institute for Infection Medicine, Christian-Albrecht University of Kiel, Kiel, Germany.

Gene Therapy
|February 19, 2010
PubMed
Summary
This summary is machine-generated.

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Human telomerase reverse transcriptase (hTERT) gene expression does not replace herpesvirus saimiri (HVS) transformation genes for T-cell amplification. Viral proteins StpC and Tip are essential for the T-cell phenotype and telomerase modulation.

Area of Science:

  • Virology
  • Molecular Biology
  • Gene Therapy

Background:

  • Herpesvirus saimiri (HVS) is a rhadinovirus used as a gene delivery vector for large DNA insertions and long-term gene expression.
  • HVS utilizes viral transformation-associated genes for T-cell transduction and amplification.

Purpose of the Study:

  • To investigate if the human telomerase reverse transcriptase (hTERT) gene can substitute for HVS transformation-associated genes in rhadinovirus-mediated T-cell transduction and amplification.
  • To determine the role of hTERT in T-cell transformation markers and growth.

Main Methods:

  • Generation of HVS mutants using en passant mutagenesis from bacterial artificial chromosomes.
  • Analysis of transgene expression, T-cell transformation markers (CD2 hyperreactivity, interleukin-26, Lyn tyrosine kinase), and growth transformation.

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Last Updated: Jun 16, 2026

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

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Published on: June 12, 2018

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  • Functional expression of transduced hTERT by the rhadinovirus vector.

    • Main Results:

    • Early and functional transgene expression was observed even in HVS mutants lacking transformation-associated genes.
    • Ectopic hTERT expression did not induce or enhance T-cell transformation markers.
    • Replacing viral transformation-associated genes with hTERT was insufficient for growth transformation, despite efficient hTERT transduction and expression.

    Conclusions:

    • The transformation-associated proteins StpC and Tip are crucial for the T-cell phenotype following HVS transduction.
    • StpC and Tip modulate telomerase activity independently of hTERT expression.
    • hTERT alone cannot substitute for the essential functions of HVS transformation genes in T-cell amplification.