Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Upsampling01:22

Upsampling

Managing signal sampling rates is essential in digital signal processing to maintain signal integrity. A decimated signal, characterized by a reduced frequency range due to its lower sampling rate, can be upsampled by inserting zeros between each sample. This upsampling process expands the original spectrum and introduces repeated spectral replicas at intervals dictated by the new Nyquist frequency. To refine this zero-inserted sequence, it is passed through a lowpass filter with a cutoff...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Downsampling01:20

Downsampling

When considering a sampled sequence with zero values between sampling instants, one can replace it by taking every N-th value of the sequence. At these integer multiples of N, the original and sampled sequences coincide. This process, known as decimation, involves extracting every N-th sample from a sequence, thereby creating a more efficient sequence.
The Fourier transform of the decimated sequence reveals a combination of scaled and shifted versions of the original spectrum. This...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Aliasing01:18

Aliasing

Accurate signal sampling and reconstruction are crucial in various signal-processing applications. A time-domain signal's spectrum can be revealed using its Fourier transform. When this signal is sampled at a specific frequency, it results in multiple scaled replicas of the original spectrum in the frequency domain. The spacing of these replicas is determined by the sampling frequency.
If the sampling frequency is below the Nyquist rate, these replicas overlap, preventing the original signal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Image approximation by variable knot bicubic splines.

IEEE transactions on pattern analysis and machine intelligence·2011
Same author

Pattern recognition experiments in the mandala/cosine domain.

IEEE transactions on pattern analysis and machine intelligence·2011
Same author

Rupture of the Bladder and Enteritis.

British medical journal·2010
Same author

Digital fourier transforms as means for scanner evaluation.

Applied optics·2010
Same author

Data compression and enhancement of sampled images.

Applied optics·2010
Same author

Computer calculated diffraction patterns.

Applied optics·2010
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
07:15

Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging

Published on: July 11, 2025

Monochrome digital image enhancement.

H C Andrews

    Applied Optics
    |February 19, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Digital computers enhance monochrome images using point and spatial processes. Techniques like nonlinear mapping and Fourier filtering improve visual quality while addressing potential artifacts in digital image enhancement.

    More Related Videos

    Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
    08:44

    Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

    Published on: June 5, 2018

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
    07:15

    Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging

    Published on: July 11, 2025

    Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
    08:44

    Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

    Published on: June 5, 2018

    Area of Science:

    • Computer Vision
    • Digital Image Processing

    Background:

    • Monochrome image enhancement is crucial for visual data analysis.
    • Digital computers offer powerful tools for image manipulation.

    Purpose of the Study:

    • To explore the application of digital computers in monochrome image enhancement.
    • To discuss various techniques for improving image quality and address potential issues.

    Main Methods:

    • Utilized point processes, including single-pixel nonlinear mappings for visual response.
    • Employed spatial processes involving linear and nonlinear filtering in Fourier and singular value decomposition domains.
    • Investigated artifact generation resulting from enhancement processes.

    Main Results:

    • Demonstrated nonlinear mappings for enhanced visual perception.
    • Showcased the effectiveness of Fourier and SVD domain filtering for image manipulation.
    • Identified and discussed artifacts that can arise during image enhancement.

    Conclusions:

    • Digital computers provide versatile methods for monochrome image enhancement.
    • A range of techniques, from simple pixel mappings to advanced filtering, can significantly improve image quality.
    • Awareness of artifact generation is essential for successful image enhancement.