Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved glycine-extracted complement-fixing antigen for human cytomegalovirus.

J D Kettering, N J Schmidt, E H Lennette

    Journal of Clinical Microbiology
    |December 1, 1977
    PubMed
    Summary

    Researchers developed a method to produce high-titered, sensitive, and stable complement-fixing antigens for human cytomegalovirus (CMV). This method involves infecting fibroblast cells and sonicating them in a specific buffer for reliable diagnostic tools.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Herpes-simplex-virus encephalitis: its possible association with reactivated latent infection.

    The New England journal of medicine·2011
    Same author

    Effect of in vitro cultivation on the pathogenicity of West Nile virus.

    Journal of bacteriology·2010
    Same author

    Effect of in vitro cultivation on the pathogenicity of West Nile virus.

    The Journal of experimental medicine·2010
    Same author

    Effect of in vitro cultivation on the pathogenicity of Venezuelan equine encephalomyelitis virus.

    The Journal of experimental medicine·2010
    Same author

    Serologic distinctness of Eastern, Western, and Venezuelan equine encephalomyelitis viruses.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same author

    Neurosecretion; cytoplasmic inclusions in peripheral autonomic ganglion cells of the monkey.

    The Anatomical record·2010

    Area of Science:

    • Virology
    • Immunology
    • Biotechnology

    Background:

    • Human cytomegalovirus (CMV) is a significant pathogen, necessitating reliable diagnostic tools.
    • Complement-fixing antigens are crucial for serological detection of CMV infections.

    Purpose of the Study:

    • To establish a consistent method for producing high-titered, sensitive, and stable complement-fixing antigens for human cytomegalovirus (CMV).

    Main Methods:

    • Human diploid fibroblast cells were infected with CMV at a high multiplicity.
    • Cells were harvested after 7 days of incubation.
    • Sonic treatment was performed immediately in 0.1 M glycine buffer (pH 9.5).

    Main Results:

    • Consistently produced high-titered antigens.

    Related Experiment Videos

  • Achieved sensitive and stable complement-fixing antigen preparations.
  • Demonstrated a reproducible method for antigen production.

    • Conclusions:

    • The described method reliably yields high-quality CMV complement-fixing antigens.
    • This production technique supports the development of sensitive and stable diagnostic assays for CMV.