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Low-temperature scanning electron microscopy in biology.

N D Read1, C E Jeffree

  • 1Institute of Cell and Molecular Biology, University of Edinburgh, U.K.

Journal of Microscopy
|January 1, 1991
PubMed
Summary
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Low-temperature scanning electron microscopy (LTSEM) offers superior specimen preservation for biological samples compared to ambient-temperature methods. This review details LTSEM protocols, experimental uses, and high-resolution advancements.

Area of Science:

  • Microscopy
  • Biophysics
  • Materials Science

Background:

  • Traditional scanning electron microscopy (SEM) often leads to specimen damage due to chemical fixation and dehydration.
  • Low-temperature scanning electron microscopy (LTSEM) preserves biological specimens closer to their native hydrated state.
  • Cryofixation rapidly immobilizes and stabilizes samples, minimizing structural alterations.

Purpose of the Study:

  • To review preparation protocols for LTSEM.
  • To highlight experimental approaches and high-resolution capabilities of LTSEM.
  • To discuss advantages and challenges of LTSEM for biological specimens.

Main Methods:

  • Review of established and recent LTSEM preparation techniques.
  • Analysis of cryofixation strategies for biological samples.

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  • Evaluation of experimental methodologies utilizing LTSEM.

    • Main Results:

    • LTSEM provides superior specimen preservation by maintaining hydration and avoiding chemical treatments.
    • Artefacts in LTSEM commonly arise from the presence of water in frozen-hydrated specimens.
    • High-resolution LTSEM represents a significant recent advancement with defined advantages and requirements.

    Conclusions:

    • LTSEM is a powerful tool for studying biological specimens with enhanced structural integrity.
    • Understanding and mitigating LTSEM-specific artefacts is crucial for accurate interpretation.
    • Further development in high-resolution LTSEM promises novel insights into cellular and molecular structures.