Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does Simpson 1 Resection of Spinal Meningiomas Lead to Lower Tumor Relapse or Increase of CSF Leaks? A 40-year Single-Center Experience.

World neurosurgery·2025
Same author

Biophysical rhizosphere processes affecting root water uptake.

Annals of botany·2016
Same author

Laboratory confirmation of C60(+) as the carrier of two diffuse interstellar bands.

Nature·2015
Same author

Correlation between erectile function and cardiovascular risk factors by assessing arterial stiffness and myocardial perfusion imaging and carotid artery intima-media thickness.

Minerva urologica e nefrologica = The Italian journal of urology and nephrology·2015
Same author

[Intestinal pseudoparasitism by Scolopendra cingulta].

Zeitschrift fur Tropenmedizin und Parasitologie·2014
Same author

Emotion work and job stressors and their effects on burnout.

Psychology & health·2012
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Scattering And Absorption of Light in Planetary Regoliths
11:34

Scattering And Absorption of Light in Planetary Regoliths

Published on: July 1, 2019

Quasi-elastic light scattering from structured particles.

S H Chen, M Holz, P Tartaglia

    Applied Optics
    |February 20, 2010
    PubMed
    Summary
    This summary is machine-generated.

    We developed a method to calculate scattered light patterns from structured particles like bacteria. This reveals internal structure through oscillatory behavior in light correlation measurements, observed in E. coli.

    More Related Videos

    Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
    09:16

    Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

    Published on: January 9, 2017

    Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
    11:34

    Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

    Published on: September 8, 2016

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Scattering And Absorption of Light in Planetary Regoliths
    11:34

    Scattering And Absorption of Light in Planetary Regoliths

    Published on: July 1, 2019

    Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
    09:16

    Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

    Published on: January 9, 2017

    Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
    11:34

    Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

    Published on: September 8, 2016

    Area of Science:

    • Optics and Photonics
    • Biophysics
    • Particle Physics

    Background:

    • Scattered light correlation functions provide insights into particle structure and dynamics.
    • Nonstationary, structured particles like bacteria present unique challenges for analysis.
    • The Rayleigh-Gans-Debye approximation is a common tool for light scattering calculations.

    Purpose of the Study:

    • To present a novel formulation for calculating the scattered field correlation function of nonstationary structured particles.
    • To investigate the relationship between internal structure and light scattering patterns.
    • To analyze the influence of diffusion and motility on scattered light spectra.

    Main Methods:

    • Development of a theoretical formulation for scattered field correlation.
    • Application of the Rayleigh-Gans-Debye approximation for micron-size bacteria.
    • Analysis of intensity correlation spectra using photon correlation techniques.
    • Experimental measurements on E. coli bacteria.

    Main Results:

    • The study demonstrates an oscillatory behavior in the intensity correlation spectrum width.
    • This oscillation is characteristic of interference patterns generated by the particle's internal structure.
    • Observed oscillatory behavior in photon correlation measurements of E. coli.

    Conclusions:

    • The proposed formulation effectively calculates scattered field correlations for structured particles.
    • Internal particle structure significantly influences light scattering spectra, leading to observable oscillations.
    • Photon correlation spectroscopy can reveal details about bacterial internal structure and dynamics.