Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
UV–Vis Spectrum01:30

UV–Vis Spectrum

When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On Treatment of Fissures of the Anus.

Western journal of medicine and surgery·2024
Same author

Political priorities and public health services in English local authorities: the case of tobacco control and smoking cessation services.

Journal of public health (Oxford, England)·2017
Same author

Primary synovial chondromatosis: a reassessment of malignant potential in 155 cases.

Skeletal radiology·2016
Same author

From mouse to man: predicting biased effects of beta-blockers in asthma.

British journal of pharmacology·2015
Same author

Relationships between impulse oscillometry, spirometry and dyspnoea in COPD.

The journal of the Royal College of Physicians of Edinburgh·2012
Same author

Prospective follow-up of novel markers of bone turnover in persistent asthmatics exposed to low and high doses of inhaled ciclesonide over 12 months.

The Journal of clinical endocrinology and metabolism·2012

Related Experiment Video

Updated: Jun 16, 2026

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
13:56

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Published on: November 28, 2012

Ultraviolet windows in commercial sunglasses.

W J Anderson, R K Gebel

    Applied Optics
    |February 20, 2010
    PubMed
    Summary

    Many nonprescription sunglasses lack adequate UV and IR protection, potentially increasing cataract risk and causing discomfort. Lens color and cost do not reliably indicate eye radiation shielding effectiveness.

    Area of Science:

    • Ophthalmology
    • Optical Physics

    Background:

    • Nonprescription sunglasses are widely used for visual comfort and perceived eye protection.
    • Concerns exist regarding the actual protective capabilities of sunglasses against harmful ultraviolet (UV) and infrared (IR) radiation.

    Purpose of the Study:

    • To evaluate the optical transmission properties of a random selection of nonprescription sunglasses.
    • To determine the extent of UV and IR radiation shielding provided by these sunglasses.
    • To identify potential risks associated with inadequate protection.

    Main Methods:

    • Optical transmission spectra were measured for a random sample of nonprescription sunglasses.
    • Measurements covered a wavelength range from 0.3 micrometers to 0.85 micrometers.
    • Transmission data was analyzed to identify UV and IR 'windows'.

    More Related Videos

    Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
    12:59

    Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

    Published on: February 15, 2020

    Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO2 Foam Transport in Fractured Unconventional Reservoirs
    10:06

    Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO2 Foam Transport in Fractured Unconventional Reservoirs

    Published on: July 2, 2020

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
    13:56

    Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

    Published on: November 28, 2012

    Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
    12:59

    Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

    Published on: February 15, 2020

    Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO2 Foam Transport in Fractured Unconventional Reservoirs
    10:06

    Microfluidic Fabrication Techniques for High-Pressure Testing of Microscale Supercritical CO2 Foam Transport in Fractured Unconventional Reservoirs

    Published on: July 2, 2020

    Main Results:

    • Several sunglass models exhibited UV windows, allowing potentially harmful UV radiation to reach the eye.
    • Over half of the tested models displayed IR windows, which may lead to discomfort during prolonged wear.
    • No correlation was found between lens color, cost, and the level of UV/IR radiation shielding.

    Conclusions:

    • Many nonprescription sunglasses offer insufficient protection against UV and IR radiation.
    • The presence of UV windows may increase the risk of cataract formation.
    • Consumers cannot rely on lens color or price to ensure adequate eye protection from radiation.