Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

IR Spectrometers

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genomic heterogeneity and clinical implications in gastrointestinal neuroendocrine carcinoma: <i>MYC</i> and <i>KRAS</i> as predictive biomarkers.

ESMO gastrointestinal oncology·2026

Same author

Embolization using hydrogel-coated coils for pulmonary arteriovenous malformations.

Diagnostic and interventional imaging·2019

Same author

Final overall survival results of WJTOG3405, a randomized phase III trial comparing gefitinib versus cisplatin with docetaxel as the first-line treatment for patients with stage IIIB/IV or postoperative recurrent EGFR mutation-positive non-small-cell lung cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2019

Same author

Short hookwire placement under imaging guidance before thoracic surgery: A review.

Diagnostic and interventional imaging·2018

Same author

Radiofrequency ablation of pulmonary tumors near the diaphragm.

Diagnostic and interventional imaging·2017

Same author

[General thoracic surgery and perioperative management in the patients with endocrine dysfunction].

Kyobu geka. The Japanese journal of thoracic surgery·2012

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Holographic interferometry with increased sensitivity for diffusely reflecting objects.

|February 20, 2010

Summary

This summary is machine-generated.

This study demonstrates a novel holographic technique for enhanced interferometry. The method amplifies phase differences, achieving higher sensitivity for deformation measurements using nonlinear holographic processing.

More Related Videos

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Related Experiment Videos

Last Updated: Jun 16, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Area of Science:

Optics and Photonics
Holography
Interferometry

Background:

Double exposure holography is a powerful tool for measuring object deformation.
Traditional holographic interferometry has limitations in sensitivity for detecting small changes.

Purpose of the Study:

To develop a nonlinear holographic method to amplify phase differences for increased interferometric sensitivity.
To experimentally validate the enhanced sensitivity using a diffusely reflecting object.

Main Methods:

Reconstruction of a double exposure hologram with simultaneous object deformation and signal beam tilt.
Nonlinear processing of the reconstructed image using a photographic plate.
Analysis of modulated Young's fringes to determine phase shifts.

Main Results:

The reconstructed image exhibited modulated Young's fringes, indicative of nonlinear holographic effects.
An interferogram with significantly increased sensitivity (lambda/16 per fringe) was achieved.
The system demonstrated effective phase difference amplification.

Conclusions:

Nonlinear processing of holographic recordings can amplify phase differences, enhancing interferometric sensitivity.
This technique offers a promising approach for high-sensitivity deformation analysis in various applications.
Further investigation into system errors is crucial for practical implementation.