Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Timing of maternal immune activation and sex influence schizophrenia-relevant cognitive constructs and neuregulin and GABAergic pathways.

Brain, behavior, and immunity·2021
Same author

Bazedoxifene - a promising brain active SERM that crosses the blood brain barrier and enhances spatial memory.

Psychoneuroendocrinology·2020
Same author

Effect of adolescent androgen manipulation on psychosis-like behaviour in adulthood in BDNF heterozygous and control mice.

Hormones and behavior·2019
Same author

Hypothalamic-pituitary-gonadal axis dysfunction: An innate pathophysiology of schizophrenia?

General and comparative endocrinology·2019
Same author

Nitrogen inputs drive nitrogen concentrations in U.S. streams and rivers during summer low flow conditions.

The Science of the total environment·2018
Same author

Posteromedial bowing of the tibia: a benign condition or a case for limb reconstruction?

Journal of children's orthopaedics·2018
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Retroreflecting multipass cell for Raman scattering.

R A Hill, A J Mulac, C E Hackett

    Applied Optics
    |February 20, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a retroreflecting multipass cell, achieving a 20x gain in Raman scattered signal intensity. This advancement is key for analyzing gas dynamics and laser systems.

    More Related Videos

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
    09:57

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

    Published on: July 25, 2022

    Observation and Analysis of Blinking Surface-enhanced Raman Scattering
    05:52

    Observation and Analysis of Blinking Surface-enhanced Raman Scattering

    Published on: January 11, 2018

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
    15:04

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

    Published on: May 18, 2011

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
    09:57

    Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

    Published on: July 25, 2022

    Observation and Analysis of Blinking Surface-enhanced Raman Scattering
    05:52

    Observation and Analysis of Blinking Surface-enhanced Raman Scattering

    Published on: January 11, 2018

    Area of Science:

    • Optics and Spectroscopy
    • Laser Physics
    • Chemical Engineering

    Background:

    • Raman spectroscopy is a powerful tool for gas analysis.
    • Multipass cells enhance signal intensity in spectroscopic measurements.
    • Efficient optical systems are needed for precise diagnostics of dynamic processes.

    Purpose of the Study:

    • To construct and evaluate a novel retroreflecting multipass cell.
    • To improve Raman scattered signal intensity for enhanced gas diagnostics.
    • To explore applications in gas dynamics and laser technology.

    Main Methods:

    • Designed and built a retroreflecting multipass cell with lenses and mirrors.
    • Tested the cell's performance in terms of signal gain and focal volume.
    • Investigated both on-axis and off-axis retroreflector configurations.

    Main Results:

    • Achieved a significant gain of 20 in Raman scattered signal intensity.
    • Demonstrated a compact focal volume of 1.1 mm x 0.3 mm x 0.3 mm.
    • Identified potential for higher gain with off-axis paraboloid systems.

    Conclusions:

    • The developed retroreflecting multipass cell effectively enhances Raman signal intensity.
    • This technology is suitable for detailed Raman diagnostics of gas dynamic processes.
    • Potential applications include flame analysis and characterization of gas mixing in laser systems.