Study of flow pattern in a fluid by scattered laser light

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Fluid dynamics is the study of fluids in motion. Velocity vectors are often used to illustrate fluid motion in applications like meteorology. For example, wind—the fluid motion of air in the atmosphere—can be represented by vectors indicating the speed and direction of the wind at any given point on a map. Another method for representing fluid motion is a streamline. A streamline represents the path of a small volume of fluid as it flows. When the flow pattern changes with time, the streamlines...

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Fluid flow analysis is critical in many scientific and engineering disciplines, and two principal approaches are used to describe this flow: the Eulerian and Lagrangian methods. These methods offer different perspectives on monitoring and analyzing the motion of fluids, each with distinct advantages depending on the scenario.
The Eulerian method focuses on fixed points in space where fluid properties, such as velocity, pressure, and temperature, are observed as the fluid moves between these...

A streamline represents the trajectory that is always tangent to the fluid's velocity vector at any given point. The velocity of a fluid particle is always directed along the streamline, ensuring the particle continuously follows the streamline's path. Streamlines are particularly useful for visualizing the overall direction of flow in a fluid system, and they provide an instantaneous representation of the flow's velocity field. In steady flow, where conditions do not change over time,...