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Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
Published on: November 1, 2017
Hematopoiesis from pluripotent stem cell lines.
Hiroshi Sakamoto1, Kiyomi Tsuji-Tamura, Minetaro Ogawa
1Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan. hisaka@kumamoto-u.ac.jp
International Journal of Hematology
|February 20, 2010
Summary
Embryonic stem cells (ESCs) and induced pluripotent stem (iPS) cells can generate hematopoietic stem cells (HSCs). Research advances using these cells offer insights into hematopoiesis and potential regenerative medicine applications.
Area of Science:
- Stem cell biology
- Hematopoiesis research
Background:
- Embryonic stem cells (ESCs) differentiate into hematopoietic progenitor cells (HPCs).
- ESC differentiation methods model early hematopoiesis and have been adapted for human ESCs.
- Induced pluripotent stem (iPS) cells offer new avenues for clinical applications in regenerative medicine.
Purpose of the Study:
- To summarize recent advancements in hematopoiesis research using ESCs and iPS cells.
- To highlight the potential of ESCs and iPS cells in regenerative medicine and transfusion applications.
Main Methods:
- Utilizing established ESC differentiation protocols.
- Adapting methods for human ESCs.
- Generating HPCs from iPS cells, including those from diseased cells.
Main Results:
- ESC differentiation protocols successfully generate various HPC lineages.
- Human ESCs show potential for regenerative medicine and transfusion therapies.
- iPS cells, including those derived from disease models, can produce phenotypically normal HPCs.
Conclusions:
- ESC and iPS cell research significantly advances our understanding of hematopoiesis.
- These pluripotent stem cell technologies hold promise for future clinical applications in treating blood disorders and enabling transfusions.
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