Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Minute-Scale Repeated Metabolic Probing of Living Cells Enabled by Rapid Parahydrogen-Based Hyperpolarization.

Angewandte Chemie (International ed. in English)·2026
Same author

Mechanistic insights into chemical exchange during the signal amplification by reversible exchange sensitization of pyruvate.

Nature communications·2026
Same author

Hyperpolarization of [1-<sup>13</sup>C]Ketoisocaproate-d<sub>2</sub> by Reversible Exchange with Parahydrogen Enables Profiling of Branched-Chain-Amino-Acid Metabolism in Cellulo and in Vivo.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

T1-weighting in Steady-State FLASH MRI-Diffusion Is Not Only Supportive but Mandatory for the Contrast.

Magnetic resonance in medicine·2026
Same author

Youth Soccer Participation and Brain Health Outcomes in Adolescent Athletes.

JAMA network open·2026
Same author

Brain regional susceptibility to tauopathy in individuals at risk for chronic traumatic encephalopathy.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026

Related Experiment Video

Updated: Jun 16, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

Fast volumetric spatial-spectral MR imaging of hyperpolarized 13C-labeled compounds using multiple echo 3D bSSFP.

William H Perman1, Pratip Bhattacharya, Jochen Leupold

  • 1Department of Radiology, Saint Louis University School of Medicine, PO Box 15250, St. Louis, MO 63110-0250, USA. perman@slu.edu

Magnetic Resonance Imaging
|February 23, 2010
PubMed
Summary

This study introduces a fast 3D chemical shift imaging technique using multiple echo 3D balanced steady state magnetic resonance imaging (ME-3DbSSFP). This method enables noninvasive measurement of hyperpolarized (13)C-labeled compounds at low concentrations.

More Related Videos

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy
08:23

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy

Published on: November 10, 2023

Related Experiment Videos

Last Updated: Jun 16, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
11:43

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents
08:59

Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents

Published on: April 15, 2016

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy
08:23

Quantitative Measure of Lung Structure and Function Obtained from Hyperpolarized Xenon Spectroscopy

Published on: November 10, 2023

Area of Science:

  • Magnetic Resonance Imaging
  • Metabolic Imaging
  • Hyperpolarized Contrast Agents

Background:

  • Noninvasive monitoring of metabolic processes is crucial in disease diagnosis.
  • Hyperpolarized (13)C-labeled compounds offer enhanced sensitivity for metabolic imaging.
  • Current techniques may lack the speed or resolution for detecting low-concentration metabolites.

Purpose of the Study:

  • To develop a rapid 3D chemical shift imaging (CSI) technique.
  • To enable noninvasive measurement of hyperpolarized (13)C-labeled substrates and metabolites.
  • To achieve detection of compounds at low concentrations.

Main Methods:

  • Utilized multiple echo 3D balanced steady state free precession (ME-3DbSSFP) imaging.
  • Performed in vitro experiments with hyperpolarized [1,3,3-2H3; 1-(13)C]2-hydroxyethylpropionate (HEP) and an acetate phantom.
  • Conducted in vivo studies on rats before and after HEP injection at 1.5 T.

Main Results:

  • Successfully generated chemical shift images of hyperpolarized HEP in vitro and in vivo.
  • Achieved isotropic 7-mm spatial resolution, 93 Hz spectral resolution, and 16-s temporal resolution.
  • Imaging was sustained for over 45 seconds, demonstrating feasibility.

Conclusions:

  • ME-3DbSSFP provides in vivo chemical shift imaging of hyperpolarized (13)C compounds.
  • The technique offers relatively high spatial and moderate spectral resolution.
  • Improved signal-to-noise ratio facilitates detection of lower concentration metabolites compared to 2D methods.