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Immune Response Against Viral Pathogens

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

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Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy

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Published on: January 12, 2024

Quantitative proteomics reveals subset-specific viral recognition in dendritic cells.

Christian A Luber¹, Jürgen Cox, Henning Lauterbach

¹Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.

|February 23, 2010

Summary

This study reveals distinct viral recognition functions in mouse dendritic cell (DC) subsets. CD8alpha(+) DCs uniquely process antigens by lacking certain cytoplasmic viral sensors, optimizing immune responses.

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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

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Last Updated: Jun 16, 2026

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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Area of Science:

Immunology
Proteomics
Cell Biology

Background:

Dendritic cells (DCs) are crucial immune orchestrators with diverse subsets.
Technological limits hinder proteome analysis of rare in vivo cell populations.

Purpose of the Study:

To comprehensively profile the proteomes of mouse splenic conventional and plasmacytoid DCs.
To identify subset-specific expression patterns of immune recognition pathways.

Main Methods:

High-resolution mass spectrometry-based proteomics.
Label-free quantitation algorithms for proteome depth.
Analysis of mouse splenic conventional and plasmacytoid DC subsets.

Main Results:

Detailed proteomes determined for conventional DCs (5,780 proteins) and plasmacytoid DCs (6,664 proteins).
Discovered mutually exclusive expression of pattern recognition pathways among DC subsets.
Assigned specific viral recognition functions exclusively to CD4(+) and double-negative DCs.
Identified that CD8alpha(+) DCs lack key cytoplasmic viral sensors.

Conclusions:

CD8alpha(+) DCs possess a unique strategy for viral antigen presentation by avoiding early activation via cytoplasmic receptors.
This mechanism allows CD8alpha(+) DCs to effectively process and present antigens before potential shutdown of antigen presentation pathways.
Findings provide new insights into the specialized roles of DC subsets in antiviral immunity.