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Related Experiment Video

Updated: Jun 16, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

Cysteine cathepsins in human dentin-pulp complex.

Ivarne L Tersariol¹, Saulo Geraldeli, Christiane L Minciotti

¹Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, São Paulo, Brazil.

Journal of Endodontics

|February 23, 2010

Summary

This summary is machine-generated.

Cysteine cathepsins and matrix metalloproteinases (MMPs) are present in dentin, contributing to age-related changes. Their activities in dentin decrease with tooth age, suggesting a role in dentin modification.

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Last Updated: Jun 16, 2026

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

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Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research

Published on: October 27, 2023

Area of Science:

Biochemistry
Oral Biology
Dentinogenesis

Background:

Odontoblasts express collagen-degrading matrix metalloproteinases (MMPs) in dentin.
Cysteine cathepsins were hypothesized to be expressed by odontoblasts and active in dentin.
Transforming growth factor beta (TGF-beta)'s effect on cathepsin expression was investigated.

Purpose of the Study:

To investigate the expression and activity of cysteine cathepsins in dentin.
To determine the role of TGF-beta in regulating cathepsin expression in odontoblasts.
To explore the relationship between cathepsin and MMP activity and tooth aging.

Main Methods:

Human odontoblasts and pulp tissue were cultured with and without TGF-beta.
Cathepsin gene expression was analyzed using DNA microarrays.
Dentin cathepsin and MMP activities were measured using fluorogenic substrates.

Main Results:

A wide range of cysteine cathepsin expression was found in odontoblasts and pulp tissue, with minor responses to TGF-beta.
Both cathepsin and MMP activities were detected in all dentin samples.
Significant negative correlations were observed between enzyme activities and tooth age.

Conclusions:

Cysteine cathepsins are present in dentin, marking the first demonstration of their presence.
Cysteine cathepsins, alongside MMPs, likely play a role in dentin modification during aging.
Enzyme activity in dentin diminishes with increasing tooth age.