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Related Concept Videos

Urinary Tract Calculi III: Medical Management01:30

Urinary Tract Calculi III: Medical Management

The diagnosis of renal calculi involves several imaging techniques, including non-contrast CT scans and ultrasound. These methods help visualize kidney stones, assess their size and location, and detect possible obstructions. Additionally, Measuring urine pH is useful for diagnosing specific stone types, such as struvite (alkaline pH) and uric acid stones (acidic pH). Cystine stones are primarily linked to cystinuria, a genetic condition. A urinalysis helps detect blood in the urine (hematuria)...
Urinary Tract Calculi V: Nursing Management01:28

Urinary Tract Calculi V: Nursing Management

AssessmentSubjective Data: Obtain a detailed health history, including any recent or chronic urinary tract infections, periods of immobilization, previous episodes of renal calculi, and medical conditions such as gout, benign prostatic hyperplasia, or hyperparathyroidism. Review the medication history for drugs that may influence stone formation, including allopurinol, analgesics, loop diuretics, or thiazide diuretics. Document the use of long-term indwelling catheters and any past surgical...
Urinary Tract Infection IV: Nursing Management01:17

Urinary Tract Infection IV: Nursing Management

In managing urinary tract infections (UTIs) in nursing, a comprehensive assessment is essential. Begin by gathering subjective data, such as the patient’s complaints of dysuria (painful urination), urinary frequency, urgency, suprapubic pain, and any lower abdominal discomfort. This information can be complemented by questions regarding previous UTIs, sexual activity, and personal hygiene practices, which can provide insight into risk factors. Objective assessment should focus on signs like...
Kidney Transplant II: Surgical Procedure01:26

Kidney Transplant II: Surgical Procedure

Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living donor...
Disorders of the Male Reproductive System01:20

Disorders of the Male Reproductive System

Men's health issues are increasingly recognized as significant, with several conditions posing common threats. Among these, testicular cancer is especially prevalent in younger men, particularly those aged 20 to 35 years. The disease often manifests as a painless mass in the testicles, sometimes accompanied by a sensation of heaviness or a dull ache.
Prostate disorders are another major concern. These conditions can impair urinary flow due to the prostate's location around the urethra. Symptoms...
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care01:30

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...

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Related Experiment Video

Updated: Jun 16, 2026

Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model
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Published on: May 29, 2015

HAMLET treatment delays bladder cancer development.

Ann-Kristin Mossberg1, Yuchuan Hou, Majlis Svensson

  • 1Department of Microbiology, Institute of Laboratory Medicine, Lund University, Lund, Sweden.

The Journal of Urology
|February 23, 2010
PubMed
Summary

Human HAMLET (a protein-lipid complex) effectively reduced bladder tumor size in mice. Topical HAMLET treatment demonstrated tumoricidal activity and may delay bladder cancer progression.

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Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model
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Area of Science:

  • Oncology
  • Biochemistry
  • Drug Delivery

Background:

  • HAMLET (a protein-lipid complex) exhibits cytotoxic effects against various cancer cells.
  • Intravesical HAMLET treatment showed promise in reducing human bladder cancer size.
  • The therapeutic potential of HAMLET in a preclinical bladder cancer model required evaluation.

Purpose of the Study:

  • To assess the therapeutic efficacy of HAMLET in the MB49 mouse bladder carcinoma model.
  • To investigate the tumoricidal and apoptotic effects of intravesical HAMLET.
  • To determine the biodistribution of HAMLET in tumor-bearing mice.

Main Methods:

  • MB49 bladder carcinoma cells were implanted in C57BL/6 mice.
  • Mice received intravesical HAMLET instillations, with controls receiving alpha-lactalbumin or buffer.
  • Tumor size, apoptosis (TUNEL staining), and drug distribution (in vivo imaging) were analyzed.

Main Results:

  • HAMLET exhibited dose-dependent cytotoxicity against MB49 cells in vitro.
  • Intravesical HAMLET significantly reduced tumor size and delayed tumor development in vivo.
  • HAMLET induced selective apoptosis in tumor tissues and showed prolonged retention in bladders of tumor-bearing mice.

Conclusions:

  • HAMLET demonstrates significant tumoricidal activity against bladder carcinoma.
  • Topical administration of HAMLET may serve as a viable strategy to delay bladder cancer progression.