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Linking desorption kinetics to phenanthrene biodegradation in soil.

Angela H Rhodes1, Laura E McAllister, Kirk T Semple

  • 1Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom.

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|February 23, 2010
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Summary

This study introduces a new method using hydroxypropyl-beta-cyclodextrin (HPCD) extraction to measure polycyclic aromatic hydrocarbons (PAHs) in soil. This technique effectively estimates the rapidly desorbing fraction, correlating well with biodegradation.

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Area of Science:

  • Environmental Chemistry
  • Soil Science
  • Biogeochemistry

Background:

  • Polycyclic aromatic hydrocarbons (PAHs) exhibit biphasic desorption kinetics, mirroring biodegradation patterns.
  • Previous methods using polymeric sorbents like Tenax are unsuitable for soil analysis.
  • Understanding PAH desorption is crucial for assessing their environmental fate and bioavailability.

Purpose of the Study:

  • To develop and validate a method for characterizing PAH desorption kinetics in soil.
  • To assess the utility of hydroxypropyl-beta-cyclodextrin (HPCD) extraction for estimating rapidly desorbing PAH fractions.
  • To establish a correlation between HPCD-extracted fractions and mass-transfer limited biodegradation.

Main Methods:

  • Desorption kinetics of (14)C-phenanthrene were measured using consecutive aqueous HPCD extractions.
  • A three-compartment model was employed to calculate the rapidly desorbing fraction (F(rap)).
  • Correlation analysis was performed between mineralized phenanthrene and the F(rap) values.

Main Results:

  • The fraction extracted by HPCD after 24 hours closely approximated the rapidly desorbing fraction (F(rap)).
  • A strong linear correlation (r(2) = 0.89) was found between phenanthrene mineralization and F(rap).
  • The model demonstrated a good fit with a gradient of 0.85 and an intercept of 8.20.

Conclusions:

  • HPCD extraction (24 h) serves as a straightforward operational tool for estimating soil PAH desorption.
  • The method accurately estimates the rapidly desorbing fraction, which is linked to biodegradation.
  • This approach facilitates the assessment of mass-transfer limited biodegradation of PAHs in soil.