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Updated: Jun 16, 2026
Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)
Published on: January 19, 2010
Brachyspira murdochii colitis in pigs
T K Jensen1, A S Christensen, M Boye
1National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark. tije@vet.dtu.dk
Abstract:
The weakly beta-hemolytic porcine spirochete Brachyspira murdochii is considered a normal intestinal commensal. In the present study, however, a field case of B murdochii-associated catarrhal colitis was identified in a pig, as characterized by extensive spirochetal colonization of the surface epithelium. Experimentally, 8 weaned pigs were challenged with the B murdochii isolate, reproducing catarrhal colitis in 2 animals. By applying fluorescent in situ hybridization using a species-specific oligonucleotide probe targeting 23S rRNA, B murdochii organisms were found in high numbers and were closely associated with the surface epithelium in the pigs with catarrhal colitis. The results indicate that, when present in high numbers, B murdochii is low pathogenic for pigs.
Insights
Brachyspira murdochii, a normal gut bacteria in pigs, can cause catarrhal colitis when present in high numbers. This study shows the low pathogenicity of this porcine spirochete.
Area of Science:
- Veterinary Microbiology
- Swine Pathology
- Bacterial Pathogenesis
Background:
- Brachyspira murdochii is typically a commensal porcine spirochete.
- Its role in intestinal disease has been unclear.
Observation:
- A field case revealed B. murdochii associated with catarrhal colitis in a pig.
- Extensive spirochetal colonization of the surface epithelium was observed.
Findings:
- Experimental challenge with B. murdochii reproduced catarrhal colitis in 2 of 8 pigs.
- High numbers of B. murdochii were found associated with the epithelium in affected animals.
- Fluorescent in situ hybridization confirmed B. murdochii presence and location.
Implications:
- Brachyspira murdochii demonstrates low pathogenicity in pigs.
- High bacterial loads are associated with the development of catarrhal colitis.
- Further research into B. murdochii's role in swine health is warranted.
