Related Experiment Video

Updated: Jun 16, 2026

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
10:05

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

LRRK2 and the stress response: interaction with MKKs and JNK-interacting proteins

C H Hsu1, D Chan, B Wolozin

  • 1Department of Pharmacology, Boston University School of Medicine, Boston, MA, USA.

Neuro-Degenerative Diseases
|February 23, 2010
PubMed

Insights

Leucine-rich repeat kinase 2 (LRRK2) interacts with scaffold proteins JIP1-4. This interaction suggests LRRK2 plays a role in regulating stress kinase signaling pathways, impacting cellular responses.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Neuroscience

Background:

  • Leucine-rich repeat kinase 2 (LRRK2) is increasingly linked to mitogen-activated protein (MAP) kinase cascades.
  • Scaffold proteins, such as JNK-interacting proteins (JIPs), are crucial for regulating stress kinase complex localization and function.

Purpose of the Study:

  • To investigate the interaction between LRRK2 and JIP scaffold proteins.
  • To elucidate the role of LRRK2 in the regulation of MAP kinase signaling pathways.

Main Methods:

  • Co-immunoprecipitation assays to detect LRRK2-JIP interactions.
  • Western blotting to analyze levels of total, oligomeric, and ubiquitinated JIP proteins.

Main Results:

  • LRRK2 was found to bind to JIP1, JIP2, JIP3, and JIP4.
  • Increased levels of total JIP1, JIP3, JIP4, oligomeric JIP, and ubiquitinated JIP were observed in association with LRRK2.
  • These findings support a role for LRRK2 in modulating JIP scaffold protein dynamics.

Conclusions:

  • LRRK2 directly interacts with JIP scaffold proteins (JIP1-4).
  • LRRK2 influences the stability and modification of JIP proteins.
  • These interactions suggest a novel regulatory mechanism for LRRK2 in stress kinase signaling cascades.

More Related Videos

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate11:31

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

Measurements of Physiological Stress Responses in C. Elegans10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...