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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

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Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
08:36

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Published on: September 6, 2011

Image patterned molecular delivery into live cells using gold particle coated substrates.

Ting-Hsiang Wu1, Sheraz Kalim, Caitlin Callahan

  • 1Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.

Optics Express
|February 23, 2010
PubMed
Summary

Researchers developed a laser-based system for precise molecular delivery into mammalian cells. This method uses patterned laser pulses on gold nanoparticles to create bubbles, enabling high-efficiency delivery of fluorescent dyes.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Precise control over molecular delivery into cells is crucial for biological research and therapeutic applications.
  • Existing methods often lack spatial resolution or efficiency in targeting specific cells or subcellular compartments.

Purpose of the Study:

  • To demonstrate an image-patterned molecular delivery system for mammalian cells.
  • To achieve high-efficiency and spatially controlled delivery of molecules using laser-induced cavitation.

Main Methods:

  • Immobilizing gold nanoparticles on a substrate positioned beneath a mammalian cell monolayer.
  • Utilizing pulsed laser irradiation to induce patterned cavitation bubble nucleation.
  • Employing a time-resolved imaging system to capture bubble dynamics.
  • Verifying molecular delivery through the uptake of a membrane-impermeable fluorescent dye (calcein).

Main Results:

  • Successful demonstration of an image-patterned molecular delivery system.
  • Observation of patterned cavitation bubble nucleation.
  • High delivery efficiency, reaching up to 90%.
  • Demonstration of multiplexed, patterned delivery of fluorescent dyes.

Conclusions:

  • The developed system offers a novel approach for precise and efficient molecular delivery into mammalian cells.
  • The technique holds potential for advanced cell-based assays, drug delivery, and genetic engineering applications.