Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026
Same author

Cancer-associated fibroblasts regulate DNA repair in pancreatic cancer through NDRG1-mediated R-loop processing.

Nature cell biology·2026
Same author

Pinhole engineering based enhanced resolution (PEER) for fluorescence lifetime imaging microscopy.

Communications biology·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026
Same author

Morphological artifacts in pulmonary pathology and literature review.

Virchows Archiv : an international journal of pathology·2026
Same author

Adaptive optics stimulated Raman scattering microscopy for topical product pharmacokinetic imaging.

Biomedical optics express·2026

Related Experiment Video

Updated: Jun 16, 2026

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
06:37

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Published on: June 15, 2022

Diffusive and directional intracellular dynamics measured by field-based dynamic light scattering.

Chulmin Joo1, Conor L Evans, Thomas Stepinac

  • 1Wellman Center for Photomedicine, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts 02114, USA.

Optics Express
|February 23, 2010
PubMed
Summary

This study introduces a novel label-free optical technique to measure intracellular dynamics. The method reveals distinct temporal regimes of cellular motion and its disruption by specific agents.

More Related Videos

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
08:17

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Related Experiment Videos

Last Updated: Jun 16, 2026

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
06:37

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Published on: June 15, 2022

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
08:17

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Area of Science:

  • Biophysics
  • Cell Biology
  • Optical Microscopy

Background:

  • Understanding intracellular dynamics is crucial for cell mechanics, function, and drug response.
  • Current methods for measuring intracellular motion can be perturbative or lack spatial resolution.

Purpose of the Study:

  • To introduce and validate a novel label-free optical technique for non-perturbative characterization of localized intracellular dynamics.
  • To investigate the temporal regimes of diffusive and directional motion within living cells.

Main Methods:

  • Combines field-based dynamic light scattering analysis with confocal interferometric microscopy.
  • Utilizes a microscopic probe volume for localized measurements.
  • Employs a label-free and non-perturbative optical approach.

Main Results:

  • Observed distinct temporal regimes of intracellular dynamics, transitioning from random to directional motion on a ~0.01 sec timescale.
  • Demonstrated disruption of directional processes (~1-5 sec) upon application of Colchicine (microtubule inhibitor) and ATP depletion.
  • Successfully characterized localized intracellular dynamics in human epithelial ovarian cancer cells.

Conclusions:

  • The developed optical technique provides a powerful tool for non-perturbative, quantitative measurement of intracellular dynamics.
  • Reveals fundamental insights into the temporal behavior of cellular processes and their modulation by external factors.
  • Offers potential applications in studying cellular responses to therapeutic agents and disease mechanisms.