Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phytochrome regulation of greening in wild type and long-hypocotyl mutants ofArabidopsis thaliana.

Planta·2013
Same author

Homologous expression of a mutated beta-tubulin gene does not confer benomyl resistance on Trichoderma virens.

Journal of applied microbiology·2003
Same author

Serotonergic dorsal raphe neurons from obese zucker rats are hyperexcitable.

Neuroscience·2003
Same author

Effects of 2G exposure on lean and genetically obese Zucker rats.

Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology·2002
Same author

Introduction: physiology, pathophysiology, and genetics of body weight/adiposity regulation.

Experimental biology and medicine (Maywood, N.J.)·2001
Same author

Meal-induced changes in extracellular 5-HT in medial hypothalamus of lean (Fa/Fa) and obese (fa/fa) Zucker rats.

Brain research·2001
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Phase image differentiation with linear intensity output.

B A Horwitz

    Applied Optics
    |February 23, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A novel square root filter enhances phase image analysis. This optical processing technique produces an output intensity directly proportional to the input phase function's derivative, aiding visualization.

    More Related Videos

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    Area of Science:

    • Optical processing
    • Image analysis
    • Digital filtering

    Background:

    • Phase imaging captures phase shifts, not intensity.
    • Processing phase images often requires specialized techniques.
    • Existing methods may lack direct proportionality for derivative extraction.

    Purpose of the Study:

    • To present a heuristic development of a square root filter.
    • To demonstrate its application in coherent optical processing for phase images.
    • To establish a direct relationship between filter output and input phase derivative.

    Main Methods:

    • Heuristic development of a square root filter.
    • Utilizing a coherent optical processor with the square root filter.
    • Applying the filter to phase image inputs.
    • Conducting digital filtering experiments for verification.

    Main Results:

    • The filter's output intensity is directly proportional to the derivative of the input phase function.
    • Digital filtering experiments confirmed this linear relationship.
    • The square root filter offers a novel approach to phase image processing.

    Conclusions:

    • The square root filter provides a direct method for obtaining the phase derivative from phase images.
    • This technique has potential applications in phase image visualization systems.
    • The demonstrated linear relationship validates the filter's utility.