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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Resonant internal-reflection prism spectroscopy using surface, guided, and Fabry-Perot EM waves.

A Hjortsberg, W P Chen, E Burstein

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    |February 23, 2010
    PubMed
    Summary

    Resonant electromagnetic modes in prisms act as powerful probes for surfaces and thin films. This technique enhances electromagnetic fields, enabling detailed optical constant analysis.

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    Area of Science:

    • Optics and Photonics
    • Surface Science
    • Spectroscopy

    Background:

    • Electromagnetic (EM) modes in multi-medium prism configurations are crucial for surface analysis.
    • Existing methods may lack sensitivity for thin film optical properties.

    Purpose of the Study:

    • To discuss resonant EM modes in three-media prisms.
    • To demonstrate their application as EM probes for surfaces, interfaces, and thin films.
    • To highlight the spectroscopic capabilities for determining optical constants.

    Main Methods:

    • Utilizing resonant internal reflection (RIR) within prism configurations.
    • Exciting surface EM, guided EM, and Fabry-Perot EM modes.
    • Analyzing the buildup of EM fields at resonant frequencies.

    Main Results:

    • Demonstrated the excitation of various resonant EM modes.
    • Showcased the significant EM field enhancement at resonant conditions.
    • Confirmed the ability to obtain optical constants of surfaces and thin overlayers.

    Conclusions:

    • Resonant EM modes in prisms offer a powerful spectroscopic tool.
    • RIR excitation provides detailed information on optical constants.
    • The method benefits from field enhancement and wavevector/frequency matching.