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Updated: Jun 16, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
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Citrinin selective molecularly imprinted polymers for SPE.

Bao-Yuan Guo1, Songxue Wang, Baozhong Ren

  • 1State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, PR China. guoby@rcees.ac.cn

Journal of Separation Science
|February 23, 2010
PubMed
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Molecularly imprinted polymers (MIPs) selectively adsorb citrinin (Cit) using a mimic template. This method accurately detects Cit in rice samples, offering reliable food safety analysis.

Area of Science:

  • Analytical Chemistry
  • Polymer Science
  • Food Safety

Background:

  • Citrinin (Cit) is a mycotoxin found in rice, posing a health risk.
  • Selective and sensitive detection methods for Cit are crucial for food safety.

Purpose of the Study:

  • To develop molecularly imprinted polymers (MIPs) for selective citrinin (Cit) detection.
  • To establish a molecularly imprinted solid-phase extraction (MISPE) method for Cit analysis in rice.

Main Methods:

  • Preparation of MIPs using 1-hydroxy-2-naphthoic acid (HNA) as a mimic template.
  • Development of a MISPE method coupled with High-Performance Liquid Chromatography (HPLC) for Cit detection.
  • Investigation of adsorption properties of MIPs and nonimprinted polymers (NIPs) for HNA and Cit.

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Main Results:

  • MIPs demonstrated significantly higher selective adsorption for HNA and Cit compared to NIPs.
  • The developed MISPE method achieved high recoveries for Cit in rice, ranging from 86.7% to 97.7%.
  • Satisfactory results were obtained when analyzing spiked and market rice samples for Cit.

Conclusions:

  • MIPs are effective for selective recognition and adsorption of citrinin.
  • The MISPE method provides a reliable and efficient approach for detecting citrinin in rice.
  • This technique contributes to enhanced food safety monitoring of mycotoxins in agricultural products.