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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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Related Experiment Video

Updated: Jun 16, 2026

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
10:57

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Published on: August 14, 2018

Transcriptome screen for fast evolving genes by Inter-Specific Selective Hybridization (ISSH).

Juan I Montoya-Burgos1, Aurélia Foulon, Ilham Bahechar

  • 1Department of Zoology and Animal Biology, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva 4, Switzerland. juan.montoya@unige.ch

BMC Genomics
|February 24, 2010
PubMed
Summary

A new Inter-Specific Selective Hybridization (ISSH) method efficiently isolates fast-evolving genes from non-model organisms. This technique enriches cDNA libraries, aiding studies in evolution and adaptation.

More Related Videos

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Related Experiment Videos

Last Updated: Jun 16, 2026

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
10:57

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Published on: August 14, 2018

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Area of Science:

  • Genomics
  • Molecular Evolution
  • Bioinformatics

Background:

  • Fast-evolving genes are crucial for understanding species adaptation and evolution.
  • Identifying these genes in non-model organisms is challenging and labor-intensive.
  • Existing methods lack efficiency for broad biological studies.

Purpose of the Study:

  • To develop and validate a novel method for enriching cDNA libraries with fast-evolving genes.
  • To facilitate the study of rapid genetic changes in non-model organisms.
  • To improve the identification of genes involved in lineage-specific processes and adaptation.

Main Methods:

  • The Inter-Specific Selective Hybridization (ISSH) method was developed.
  • It utilizes homologous tissue transcripts from distinct but related species.
  • Stringency conditions select for heteroduplexes that disanneal at low temperatures, enriching for fast-evolving sequences.

Main Results:

  • The ISSH method successfully generated a brain cDNA library enriched in fast-evolving transcripts from a non-model catfish.
  • Enriched libraries showed a higher proportion of fast-evolving sequences compared to control libraries.
  • Gene annotation revealed enrichment in low-expression and non-ubiquitous genes, characteristic of fast-evolving genes.

Conclusions:

  • The ISSH method provides a simple, cost-effective, and efficient approach for isolating fast-evolving genes.
  • This technique enhances the investigation of evolutionary mechanisms, including lineage-specific adaptations.
  • It opens new avenues for research in population genetics and evolutionary biology.