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Related Experiment Video

Updated: Jun 16, 2026

Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells
06:21

Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells

Published on: August 27, 2019

KSHV-infected PEL cell lines exhibit a distinct gene expression profile.

Keiji Ueda1, Emi Ito, Masato Karayama

  • 1Division of Virology, Department of Microbiology and Immunology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan. kueda@virus.med.osaka-u.ac.jp

Biochemical and Biophysical Research Communications
|February 24, 2010
PubMed
Summary

Gene expression profiling identified distinct molecular signatures for primary effusion lymphoma (PEL), T-cell leukemia (TCL), and Burkitt lymphoma (BL) cell lines. These profiles, influenced by viruses like KSHV, aid in classifying these lymphocyte-derived tumors.

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Area of Science:

  • Oncology
  • Virology
  • Genomics

Background:

  • Lymphocyte-derived tumors, including primary effusion lymphoma (PEL), T-cell leukemia (TCL), and Burkitt lymphoma (BL), exhibit diverse molecular characteristics.
  • Understanding the gene expression profiles of these cell lines is crucial for accurate classification and identifying potential viral influences.

Purpose of the Study:

  • To determine characteristic gene expression profiles for PEL, TCL, and BL cell lines.
  • To compare these profiles with normal peripheral blood mononuclear cells (PBMCs).
  • To investigate the impact of viral co-infections on gene expression in tumor cell lines.

Main Methods:

  • Analysis of gene expression profiles using transcriptomic techniques.
  • Comparison of gene expression patterns across different tumor cell lines and normal PBMCs.
  • Statistical analysis to identify distinct and characteristic gene expression signatures.

Main Results:

  • PEL, TCL, and BL cell lines demonstrated distinct gene expression profiles, allowing clear classification into four groups: PEL, TCL, BL, and normal PBMCs.
  • B lymphocyte-derived tumor cell lines (PEL and BL) showed separate gene expression signatures.
  • A cell line co-infected with Kaposi's sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV) showed a gene expression profile predominantly governed by KSHV.

Conclusions:

  • Established tumor cell lines possess unique, identifiable gene expression profiles.
  • These distinct profiles can aid in the classification and understanding of lymphocyte-derived malignancies.
  • Viral infections, such as KSHV, can significantly influence the gene expression landscape of tumor cells.