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Updated: Jun 16, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
Unprecedented NES non-antagonistic inhibitor for nuclear export of Rev from Sida cordifolia
Satoru Tamura1, Masafumi Kaneko, Atsushi Shiomi
1Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
Abstract:
Bioassay-guided separation from the MeOH extract of the South American medicinal plant Sida cordifolia resulted in isolation of (10E,12Z)-9-hydroxyoctadeca-10,12-dienoic acid (1) as an unprecedented NES non-antagonistic inhibitor for nuclear export of Rev. This mechanism of action was established by competitive experiment by the biotinylated probe derived from leptomycin B, the known NES antagonistic inhibitor. Additionally, structure-activity relationship analysis by use of the synthesized analogs clarified cooperation of several functionalities in the Rev-export inhibitory activity of 1.
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