Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Imaging vocal fold medial surface vibration across chest, head, whistle, and creaky voice production using optical coherence tomography: A single subject study.

JASA express letters·2026

Same author

Quantifying Vocal Fold Vibration in the Coronal Plane Using Optical Coherence Tomography in Normal Human Subjects.

Journal of voice : official journal of the Voice Foundation·2026

Same author

Diagnosing Eosinophilic Chronic Rhinosinusitis in Excised Tissue Using Optical Coherence Tomography.

The Laryngoscope·2025

Same author

Multimodal microscope for optical coherence microscopy, tomography, vibrometry, and two-photon microscopy in the living mouse cochlea.

Journal of biomedical optics·2025

Same author

Optical coherence tomography-based cochlear endoscopy through the human round window membrane.

Optics letters·2025

Same author

Optical coherence tomography imaging demonstrates endolymphatic hydrops in the lateral and posterior semicircular canals in noise-exposed mice.

Hearing research·2025

Same journal

Highly Accelerated 1-mm Isotropic 3D Chemical Exchange Saturation Transfer MRI Using Wave-Co-CAIPI at 5 Tesla.

IEEE transactions on bio-medical engineering·2026

Same journal

Systematic Evaluation of Hip Exoskeleton Assistance Parameters for Enhancing Gait Stability During Ground Slip Perturbations.

IEEE transactions on bio-medical engineering·2026

Same journal

SleepConFormer: A Single-Channel EEG Framework for Sleep Staging and Consciousness Assessment in Patients with Disorders of Consciousness.

IEEE transactions on bio-medical engineering·2026

Same journal

Modeling Partial and Total Support of Left Ventricular Assist Device for Discrete Hemodynamic Control Framework.

IEEE transactions on bio-medical engineering·2026

Same journal

A Low-Cost Wearable TI-TACS Stimulator With Bipolar Quadratic-Boost Converter for Current Stimulation Validation in the Rat Brain.

IEEE transactions on bio-medical engineering·2026

Same journal

EMG-Based Gait Estimation Using Koopman-Inspired Method.

IEEE transactions on bio-medical engineering·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Off-axis photoacoustic microscopy.

Ryan L Shelton¹, Brian E Applegate

¹Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA. shelton8r@gmail.com

IEEE Transactions on Bio-Medical Engineering

|February 24, 2010

Summary

This summary is machine-generated.

We developed a simplified photoacoustic microscopy (PAM) system for high-contrast imaging of hemoglobin and melanin. This novel design improves implementation and can be adapted for various microscopy applications.

More Related Videos

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Related Experiment Videos

Last Updated: Jun 16, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Area of Science:

Biomedical optics
Medical imaging
Photoacoustic microscopy

Background:

Photoacoustic microscopy (PAM) offers high-contrast, high-resolution imaging, crucial for visualizing hemoglobin and melanin.
Applications include mapping microvasculature and melanoma tumor margins.

Purpose of the Study:

To present a novel, simplified photoacoustic microscopy design.
To enable the use of commercial optics and transducers, enhancing adaptability to existing microscopes.

Main Methods:

A new PAM design separates optical illumination from acoustic detection.
An off-axis PAM system was assembled and characterized using tissue samples.
The system achieves a lateral resolution of 26 micrometers and axial resolution of 410 micrometers.

Main Results:

The simplified design allows for high-quality commercial component integration.
The system provides quasi-dark-field detection due to signal sensitivity in the overlap of illumination and detection solid angles.
Axial resolution can be scaled to tens of micrometers using high-frequency acoustic transducers.

Conclusions:

The novel PAM design simplifies implementation and enhances adaptability.
This approach facilitates high-resolution imaging of biological tissues.
The system holds potential for improved diagnostic and research applications in biomedical imaging.