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Updated: Jun 16, 2026
Pentylenetetrazole-Induced Kindling Mouse Model
Published on: June 12, 2018
Reversal of pentylenetetrazole-induced seizure activity in mice by nickel chloride
1Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala - 147 002, India.
Objective:
The present study was designed to investigate the anticonvulsant potential of nickel which is shown to selectively block t-type calcium channels by using nickel choride on pentylenetetrazole (80 mg/kg) induced seizure activity model in mice.
Materials And Methods:
Seizures were assessed in terms of onset of Straub's tail phenomenon and onset of jerky movements of the whole body, convulsions, and death. Sodium valproate served as a standard control in the present study.
Results:
Nickel chloride (5 mg/kg i.p. and 10 mg/kg i.p.) attenuated pentylenetetrazole-induced seizure activity in mice, as reflected by a significant increase in the onset time of Straub's tail phenomenon and onset of jerky movements of the whole body, convulsions, and death. High dose of nickel chloride showed more pronounced anticonvulsant action than sodium valproate.
Conclusions:
The anticonvulsant action of nickel chloride was noticeable in this study. However, further studies are required to elucidate its full anticonvulsant potential.
Insights
Nickel chloride demonstrated anticonvulsant potential by blocking t-type calcium channels, significantly reducing pentylenetetrazole-induced seizures in mice. Further research is needed to explore its full therapeutic capabilities.
Area of Science:
- Neuropharmacology
- Calcium Channel Blockade
Background:
- T-type calcium channels play a role in seizure generation.
- Nickel is known to selectively block these channels.
Purpose of the Study:
- To investigate the anticonvulsant potential of nickel chloride.
- To evaluate its efficacy in a pentylenetetrazole-induced seizure model in mice.
Main Methods:
- Mice were administered nickel chloride (5 mg/kg and 10 mg/kg).
- Seizure activity was induced using pentylenetetrazole (80 mg/kg).
- Onset of seizure phenomena (Straub's tail, jerky movements, convulsions, death) was recorded. Sodium valproate was used as a control.
Main Results:
- Nickel chloride administration significantly increased the onset time of seizure phenomena.
- Both tested doses of nickel chloride showed anticonvulsant effects.
- The higher dose of nickel chloride exhibited a more pronounced effect than sodium valproate.
Conclusions:
- Nickel chloride possesses noticeable anticonvulsant properties.
- The findings suggest nickel chloride's potential as an anticonvulsant agent.
- Further studies are warranted to fully elucidate its therapeutic potential.
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