Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 15, 2026

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Published on: April 19, 2021

A microfluidic approach for high-throughput droplet interface bilayer (DIB) formation.

C E Stanley¹, K S Elvira, X Z Niu

¹Department of Chemistry, Imperial College London, Exhibition Road, South Kensington, London, UKSW7 2AZ.

Chemical Communications (Cambridge, England)

|February 24, 2010

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Droplet microfluidics for the construction of compartmentalised model membranes.

Lab on a chip·2018

Same author

Self-assembled materials and supramolecular chemistry within microfluidic environments: from common thermodynamic states to non-equilibrium structures.

Chemical Society reviews·2018

Same author

Electrospraying of microfluidic encapsulated cells for the fabrication of cell-laden electrospun hybrid tissue constructs.

Acta biomaterialia·2017

Same author

Monitoring plasmon coupling and SERS enhancement through in situ nanoparticle spacing modulation.

Faraday discussions·2017

Same author

From [<sup>11</sup>C]CO<sub>2</sub> to [<sup>11</sup>C]amides: a rapid one-pot synthesis via the Mitsunobu reaction.

Chemical communications (Cambridge, England)·2017

Same author

Engineering plant membranes using droplet interface bilayers.

Biomicrofluidics·2017

We developed an automated microfluidic method for rapidly creating droplet interface bilayers (DIBs). This technique allows for the construction of complex DIB networks within 3D structures using various lipids.

Area of Science:

Biophysics
Materials Science
Microfluidics

Background:

Droplet interface bilayers (DIBs) are crucial for constructing artificial cell membranes.
High-throughput and automated methods are needed to advance DIB applications.

Purpose of the Study:

To present a simple, automated microfluidic method for high-throughput DIB formation.
To demonstrate the capability of forming complex DIB networks in 3D architectures.
To showcase the system's flexibility with different lipid compositions.

Main Methods:

Utilized a microfluidic device for automated droplet handling.
Engineered a system for sequential droplet merging to form DIBs.
Employed various lipids, including DPhPC and DOPC, for bilayer formation.

More Related Videos

Bilayer Microfluidic Device for Combinatorial Plug Production

Bilayer Microfluidic Device for Combinatorial Plug Production

Published on: December 1, 2023

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Related Experiment Videos

Last Updated: Jun 15, 2026

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Published on: April 19, 2021

Bilayer Microfluidic Device for Combinatorial Plug Production

Bilayer Microfluidic Device for Combinatorial Plug Production

Published on: December 1, 2023

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

Main Results:

Successfully achieved high-throughput formation of DIBs.
Demonstrated the ability to create complex DIB networks.
Showcased the filling of predefined 3D architectures with DIB networks.
Confirmed successful DIB formation using different lipid types.

Conclusions:

The developed microfluidic method offers a simple and automated approach for high-throughput DIB formation.
This system enables the construction of intricate DIB networks within 3D scaffolds.
The flexibility in lipid choice highlights the potential for diverse applications in synthetic biology and materials science.