Related Experiment Video
Updated: Aug 1, 2026

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
Published on: January 16, 2015
Effects of 2,4-dichlorophenoxyacetic acid on polyamine synthesis in Chinese hamster ovary cells
1Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Abstract:
It was found that 1 mM 2,4-dichlorophenoxyacetate (2,4-D) inhibited DNA and protein synthesis in Chinese hamster ovary (CHO) cells. When the possible relationship of this phenomenon to the presence of polyamines in the culture medium was investigated, it was found that: (a) the pesticide inhibited ornithine decarboxylase activity; (b) when the concentration of polyamines present in cells treated with the pesticide was determined, the putrescine concentration did not change, and the spermine and spermidine concentration decreased; (c) the addition of spermidine and spermine to CHO cells grown in the presence of 2,4-D normalized DNA and protein synthesis. Putrescine did not have any effect.
Related Concept Videos
Preparation of 1° Amines: Gabriel Synthesis
Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...
Direct-Acting Cholinergic Agonists: Pharmacokinetics
Anticholinesterase Agents: Poisoning and Treatment
Irreversible agents form a strong bond with the cholinesterase enzyme, making it inactive. The breakdown of the phosphorylated enzyme is slower than the...

