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Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...

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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Published on: June 6, 2017

Timing is everything: cell cycle control of Rad52.

Jacqueline H Barlow1, Rodney Rothstein

  • 1Department of Genetics & Development, Columbia University Medical Center, 701 West 168th Street, HHSC 1608, New York, NY 10032-2704, USA. rothstein@cancercenter.columbia.edu.

Cell Division
|February 25, 2010
PubMed
Summary

DNA double-strand break repair relies on regulating the Rad52 protein in yeast. Proper timing and modification of Rad52 ensure cell viability and genomic stability during DNA repair.

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Last Updated: Jun 15, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Homologous recombination is crucial for repairing DNA double-strand breaks, maintaining cell viability and genomic integrity.
  • The protein Rad52 is central to homologous recombination in Saccharomyces cerevisiae, mediating strand exchange and DNA annealing.
  • Regulation of Rad52 recruitment to DNA lesions is key to controlling repair processes.

Discussion:

  • Rad52 activity is tightly controlled throughout the cell cycle, including positive regulation during S phase entry and repression during the intra-S phase checkpoint.
  • Posttranslational modifications, such as phosphorylation and sumoylation, significantly impact Rad52's stability, recruitment timing, and overall function.
  • Understanding these regulatory mechanisms is vital for comprehending DNA repair fidelity and cellular responses to DNA damage.

Key Insights:

  • The timing of Rad52 recruitment to DNA damage sites is a critical regulatory step in homologous recombination.
  • Posttranslational modifications of Rad52 are essential for its proper function in DNA double-strand break repair.
  • Controlled Rad52 regulation directly influences yeast cell survival and the maintenance of genomic integrity.

Outlook:

  • Further research into Rad52 regulation could reveal new therapeutic targets for diseases associated with DNA repair defects.
  • Investigating conserved regulatory mechanisms across different species may provide broader insights into genome stability.
  • Exploring the interplay between cell cycle checkpoints and DNA repair protein regulation offers avenues for understanding cancer biology.