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Conserved nucleotide sequence of rabies virus cDNA encoding the nucleoprotein
K Mannen1, K Hiramatsu, K Mifune
1Department of Microbiology, Medical College of Oita, Japan.
Virus Genes
|January 1, 1991
Summary
The rabies virus nucleoprotein gene is highly conserved across different strains. This finding is crucial for understanding rabies virus evolution and developing better diagnostics and vaccines.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Rabies virus causes a fatal neurological disease in mammals.
- Understanding the genetic diversity of rabies virus is essential for disease control.
- Structural proteins play key roles in viral replication and pathogenesis.
Purpose of the Study:
- To clone and sequence the complementary DNA (cDNA) encoding structural proteins of the rabies virus (CVS strain).
- To determine the nucleotide sequence of the rabies virus nucleoprotein (N) gene.
- To compare the N gene sequence with those of other rabies virus strains.
Main Methods:
- Cloning of complementary DNA (cDNA) encoding rabies virus structural proteins (G, N, NS, M).
- Nucleotide sequencing of the cloned cDNA for the nucleoprotein (N).
- Bioinformatic analysis to compare nucleotide and amino acid sequences.
Main Results:
- Complementary DNA (cDNA) encoding the G, N, NS, and M structural proteins of the rabies virus (CVS strain) were successfully cloned.
- The nucleotide sequence of the rabies virus nucleoprotein (N) gene was determined.
- Sequence comparison revealed high conservation of nucleotide and deduced amino acid sequences among different rabies virus strains, including an avirulent strain.
Conclusions:
- The nucleoprotein gene of the rabies virus exhibits significant sequence conservation across various strains.
- This high degree of conservation suggests a critical functional role for the nucleoprotein.
- Findings contribute to the understanding of rabies virus genetic stability and evolution.