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Related Experiment Video

Updated: Jun 15, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

New baculovirus expression tools for recombinant protein complex production.

Simon Trowitzsch¹, Christoph Bieniossek, Yan Nie

¹European Molecular Biology Laboratory (EMBL), Grenoble Outstation, and Unit of Virus Host Cell Interactions UVHCI, UMI3265, 6 rue Jules Horowitz, Grenoble Cedex 9, France.

Journal of Structural Biology

|February 25, 2010

Summary

Producing large protein complexes for research is challenging. We developed the MultiBac system to streamline recombinant protein production and diversification in insect cells, simplifying structural studies.

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Last Updated: Jun 15, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

The MultiBac Protein Complex Production Platform at the EMBL

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay

Published on: February 22, 2018

Area of Science:

Molecular Biology
Structural Biology
Biochemistry

Background:

Eukaryotic proteins often function as large, multicomponent assemblies.
Low native abundance of these molecular machines hinders purification and structural analysis.
Heterologous overproduction is crucial for high-resolution structure determination.

Purpose of the Study:

To present technologies for streamlining recombinant multiprotein complex production.
To review developments in the MultiBac baculovirus/insect cell expression system.
To detail improvements in baculovirus expression system performance for complex protein production.

Main Methods:

Utilized the MultiBac baculovirus/insect cell expression system.
Developed robotic routines for automated multigene vector generation.
Modified transfer plasmids and generated composite multigene baculoviral DNA.

Main Results:

Streamlined the complex production and diversification process for multiprotein complexes.
Achieved parallelization and automation of gene assembly for protein complex expression.
Introduced simplified and standardized expression procedures using the MultiBac system.

Conclusions:

The developed technologies facilitate efficient recombinant multiprotein complex production.
Improvements to the MultiBac system enhance baculovirus expression performance.
These advancements aid in the structural and functional studies of challenging protein assemblies.