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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: Jun 15, 2026

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Published on: February 24, 2015

Whole methylome analysis by ultra-deep sequencing using two-base encoding.

Christina A Bormann Chung1, Victoria L Boyd, Kevin J McKernan

  • 1Life Technologies, Foster City, California, United States of America. Christina.Chung@lifetech.com

Plos One
|February 25, 2010
PubMed
Summary
This summary is machine-generated.

This study compares two bisulfite conversion methods for whole methylome sequencing using SOLiD technology. It discusses the pros and cons of each method for DNA sequencing library construction.

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Area of Science:

  • Epigenetics
  • Genomics
  • Molecular Biology

Background:

  • DNA methylation regulates gene expression in normal and diseased cells.
  • Bisulfite conversion is key to detecting DNA methylation patterns.
  • Next-generation sequencing (NGS) enables whole methylome analysis.

Purpose of the Study:

  • To compare two bisulfite conversion methods for whole methylome sequencing.
  • To evaluate the suitability of these methods for high-throughput sequencing platforms like SOLiD.
  • To discuss the advantages and disadvantages of each method for library construction.

Main Methods:

  • Whole methylome sequencing using the SOLiD System.
  • Comparison of two bisulfite conversion techniques: in solution and in gel.
  • Analysis of bisulfite-converted DNA libraries for sequencing.

Main Results:

  • The study evaluates the performance of in-solution versus in-gel bisulfite conversion for SOLiD sequencing.
  • Advantages and disadvantages of each method for library preparation are detailed.
  • Preliminary in silico analysis demonstrates potential application to complex genomes.

Conclusions:

  • Both bisulfite conversion methods are viable for whole methylome sequencing.
  • Method selection depends on specific research needs and sequencing platform.
  • SOLiD bisulfite sequencing shows promise for analyzing complex methylomes.